Title |
RNA-guided CRISPR-Cas technologies for genome-scale investigation of disease processes
|
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Published in |
Journal of Hematology & Oncology, April 2015
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DOI | 10.1186/s13045-015-0127-3 |
Pubmed ID | |
Authors |
Sean E Humphrey, Andrea L Kasinski |
Abstract |
From its discovery as an adaptive bacterial and archaea immune system, the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has quickly been developed into a powerful and groundbreaking programmable nuclease technology for the global and precise editing of the genome in cells. This system allows for comprehensive unbiased functional studies and is already advancing the field by revealing genes that have previously unknown roles in disease processes. In this review, we examine and compare recently developed CRISPR-Cas platforms for global genome editing and examine the advancements these platforms have made in guide RNA design, guide RNA/Cas9 interaction, on-target specificity, and target sequence selection. We also explore some of the exciting therapeutic potentials of the CRISPR-Cas technology as well as some of the innovative new uses of this technology beyond genome editing. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 17% |
France | 1 | 17% |
Unknown | 4 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 83% |
Scientists | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 1% |
Portugal | 1 | 1% |
Unknown | 89 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 18 | 20% |
Researcher | 16 | 18% |
Student > Ph. D. Student | 14 | 15% |
Student > Master | 12 | 13% |
Student > Postgraduate | 7 | 8% |
Other | 17 | 19% |
Unknown | 7 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 38 | 42% |
Biochemistry, Genetics and Molecular Biology | 32 | 35% |
Medicine and Dentistry | 4 | 4% |
Neuroscience | 3 | 3% |
Immunology and Microbiology | 2 | 2% |
Other | 3 | 3% |
Unknown | 9 | 10% |