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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice
|
|---|---|
| Published in |
Genome Biology, April 2015
|
| DOI | 10.1186/s13059-015-0653-x |
| Pubmed ID | |
| Authors |
Tomomi Aida, Keiho Chiyo, Takako Usami, Harumi Ishikubo, Risa Imahashi, Yusaku Wada, Kenji F Tanaka, Tetsushi Sakuma, Takashi Yamamoto, Kohichi Tanaka |
| Abstract |
Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target digestion, leading to generation of knock-in mice carrying a functional cassette with up to 50% efficiency, compared with just 10% by a commonly used method consisting of Cas9 mRNA and single guide RNA. Our cloning-free CRISPR/Cas system facilitates rapid one-step generation of cassette knock-in mice, accelerating functional genomic research by providing various in vivo genetic tools. |
X Demographics
The data shown below were collected from the profiles of 32 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 4 | 13% |
| United States | 4 | 13% |
| Japan | 3 | 9% |
| Canada | 1 | 3% |
| Australia | 1 | 3% |
| France | 1 | 3% |
| Germany | 1 | 3% |
| Unknown | 17 | 53% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 23 | 72% |
| Scientists | 7 | 22% |
| Science communicators (journalists, bloggers, editors) | 2 | 6% |
Mendeley readers
The data shown below were compiled from readership statistics for 508 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 7 | 1% |
| United Kingdom | 3 | <1% |
| Switzerland | 2 | <1% |
| United States | 2 | <1% |
| Spain | 2 | <1% |
| South Africa | 1 | <1% |
| Finland | 1 | <1% |
| Chile | 1 | <1% |
| Slovenia | 1 | <1% |
| Other | 3 | <1% |
| Unknown | 485 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 134 | 26% |
| Student > Ph. D. Student | 102 | 20% |
| Student > Master | 50 | 10% |
| Student > Bachelor | 44 | 9% |
| Professor > Associate Professor | 30 | 6% |
| Other | 78 | 15% |
| Unknown | 70 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 194 | 38% |
| Biochemistry, Genetics and Molecular Biology | 146 | 29% |
| Medicine and Dentistry | 31 | 6% |
| Neuroscience | 31 | 6% |
| Immunology and Microbiology | 9 | 2% |
| Other | 18 | 4% |
| Unknown | 79 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 54. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2023.
All research outputs
#800,324
of 25,959,914 outputs
Outputs from Genome Biology
#527
of 4,519 outputs
Outputs of similar age
#9,432
of 281,845 outputs
Outputs of similar age from Genome Biology
#10
of 71 outputs
Altmetric has tracked 25,959,914 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,519 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.7. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,845 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 71 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.