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Establishment of a patient-derived orthotopic osteosarcoma mouse model

Overview of attention for article published in Journal of Translational Medicine, April 2015
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Title
Establishment of a patient-derived orthotopic osteosarcoma mouse model
Published in
Journal of Translational Medicine, April 2015
DOI 10.1186/s12967-015-0497-x
Pubmed ID
Authors

Claudia Blattmann, Markus Thiemann, Albrecht Stenzinger, Eva K Roth, Anne Dittmar, Hendrik Witt, Burkhard Lehner, Eva Renker, Manfred Jugold, Viktoria Eichwald, Wilko Weichert, Peter E Huber, Andreas E Kulozik

Abstract

Osteosarcoma (OS) is the most common pediatric primary malignant bone tumor. As the prognosis for patients following standard treatment did not improve for almost three decades, functional preclinical models that closely reflect important clinical cancer characteristics are urgently needed to develop and evaluate new treatment strategies. The objective of this study was to establish an orthotopic xenotransplanted mouse model using patient-derived tumor tissue. Fresh tumor tissue from an adolescent female patient with osteosarcoma after relapse was surgically xenografted into the right tibia of 6 immunodeficient BALB/c Nu/Nu mice as well as cultured into medium. Tumor growth was serially assessed by palpation and with magnetic resonance imaging (MRI). In parallel, a primary cell line of the same tumor was established. Histology and high-resolution array-based comparative genomic hybridization (aCGH) were used to investigate both phenotypic and genotypic characteristics of different passages of human xenografts and the cell line compared to the tissue of origin. A primary OS cell line and a primary patient-derived orthotopic xenotranplanted mouse model were established. MRI analyses and histopathology demonstrated an identical architecture in the primary tumor and in the xenografts. Array-CGH analyses of the cell line and all xenografts showed highly comparable patterns of genomic progression. So far, three further primary patient-derived orthotopic xenotranplanted mouse models could be established. We report the first orthotopic OS mouse model generated by transplantation of tumor fragments directly harvested from the patient. This model represents the morphologic and genomic identity of the primary tumor and provides a preclinical platform to evaluate new treatment strategies in OS.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Unknown 66 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 18%
Student > Ph. D. Student 12 18%
Student > Master 9 13%
Student > Postgraduate 6 9%
Student > Bachelor 5 7%
Other 11 16%
Unknown 12 18%
Readers by discipline Count As %
Medicine and Dentistry 27 40%
Biochemistry, Genetics and Molecular Biology 5 7%
Nursing and Health Professions 3 4%
Agricultural and Biological Sciences 3 4%
Engineering 3 4%
Other 12 18%
Unknown 14 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2015.
All research outputs
#15,331,767
of 22,803,211 outputs
Outputs from Journal of Translational Medicine
#2,233
of 3,991 outputs
Outputs of similar age
#156,676
of 263,976 outputs
Outputs of similar age from Journal of Translational Medicine
#58
of 91 outputs
Altmetric has tracked 22,803,211 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,991 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.5. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,976 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 91 others from the same source and published within six weeks on either side of this one. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.