Title |
Intracellular trafficking as a determinant of AS-DACA cytotoxicity in rhabdomyosarcoma cells
|
---|---|
Published in |
BMC Molecular and Cell Biology, August 2011
|
DOI | 10.1186/1471-2121-12-36 |
Pubmed ID | |
Authors |
Steven J Wolf, Tony Huynh, Nicole S Bryce, Trevor W Hambley, Laurence PG Wakelin, Bernard W Stewart, Daniel R Catchpoole |
Abstract |
Rhabdomyosarcoma (RMS) is a malignant soft tissue sarcoma derived from skeletal muscle precursor cells, which accounts for 5-8% of all childhood malignancies. Disseminated RMS represents a major clinical obstacle, and the need for better treatment strategies for the clinically aggressive alveolar RMS subtype is particularly apparent. Previously, we have shown that the acridine-4-carboxamide derivative AS-DACA, a known topoisomerase II poison, is potently cytotoxic in the alveolar RMS cell line RH30, but is 190-fold less active in the embryonal RMS cell line RD. Here, we investigate the basis for this selectivity, and demonstrate in these RMS lines, and in an AS-DACA- resistant subclone of RH30, that AS-DACA-induced cytotoxicity correlates with the induction of DNA double strand breaks. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 4% |
Unknown | 24 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 20% |
Student > Bachelor | 3 | 12% |
Student > Ph. D. Student | 3 | 12% |
Professor > Associate Professor | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 4 | 16% |
Unknown | 6 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 24% |
Medicine and Dentistry | 5 | 20% |
Agricultural and Biological Sciences | 3 | 12% |
Chemistry | 2 | 8% |
Psychology | 1 | 4% |
Other | 1 | 4% |
Unknown | 7 | 28% |