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Variable expression levels of keratin and vimentin reveal differential EMT status of circulating tumor cells and correlation with clinical characteristics and outcome of patients with metastatic…

Overview of attention for article published in BMC Cancer, May 2015
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Title
Variable expression levels of keratin and vimentin reveal differential EMT status of circulating tumor cells and correlation with clinical characteristics and outcome of patients with metastatic breast cancer
Published in
BMC Cancer, May 2015
DOI 10.1186/s12885-015-1386-7
Pubmed ID
Authors

Hara Polioudaki, Sofia Agelaki, Rena Chiotaki, Eleni Politaki, Dimitris Mavroudis, Alexios Matikas, Vassilis Georgoulias, Panayiotis A Theodoropoulos

Abstract

CTCs expressing variable levels of epithelial and mesenchymal markers in breast cancer have previously been reported. However, no information exists for keratin expression levels of CTCs in association with disease status, whereas assays for the characterization of transitional EMT phenotypes of CTCs in breast cancer are rather lacking. We investigated the correlation between keratin expression of CTCs and patients' outcome and characterized the EMT status of CTCs via the establishment of a numerical "ratio" value of keratin and vimentin expression levels on a single cell basis. Keratin expression was evaluated in 1262 CTCs from 61 CTC-positive patients with metastatic breast cancer, using analysis of images obtained through the CellSearch System. For the determination of vimentin/keratin (vim/K) ratios, expression levels of keratin and vimentin were measured in cytospin preparations of luminal (MCF-7 and T47D) and basal (MDA.MB231 and Hs578T) breast cancer cell lines and 110 CTCs from 5 CTC-positive patients using triple immunofluorescence laser scanning microscopy and image analysis. MCF-7 and T47D displayed lower vim/K ratios compared to MDA.MB231 and Hs578T cells, while MCF-7 cells that had experimentally undergone EMT were characterized by varying intermediate vim/K ratios. CTCs were consisted of an heterogeneous population presenting variable vim/K values with 46% of them being in the range of luminal breast cancer cell lines. Keratin expression levels of CTCs detected by the CellSearch System correlated with triple negative (p = 0.039) and ER-negative (p = 0.025) breast cancer, and overall survival (p = 0.038). Keratin expression levels of CTCs correlate with tumor characteristics and clinical outcome. Moreover, CTCs display significant heterogeneity in terms of the degree of EMT phenotype that probably reflects differential invasive potential. The assessment of the vim/K ratios as a surrogate marker for the EMT status of CTCs merits further investigation as a prognostic tool in breast cancer.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
Ukraine 1 1%
Unknown 86 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 22%
Student > Master 15 17%
Researcher 14 16%
Student > Bachelor 12 14%
Student > Postgraduate 7 8%
Other 9 10%
Unknown 12 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 23 26%
Agricultural and Biological Sciences 19 22%
Medicine and Dentistry 12 14%
Pharmacology, Toxicology and Pharmaceutical Science 3 3%
Immunology and Microbiology 3 3%
Other 10 11%
Unknown 18 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 May 2015.
All research outputs
#3,574,363
of 5,090,406 outputs
Outputs from BMC Cancer
#1,794
of 2,824 outputs
Outputs of similar age
#116,804
of 161,909 outputs
Outputs of similar age from BMC Cancer
#150
of 187 outputs
Altmetric has tracked 5,090,406 research outputs across all sources so far. This one is in the 16th percentile – i.e., 16% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,824 research outputs from this source. They receive a mean Attention Score of 2.6. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
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We're also able to compare this research output to 187 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.