Title |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area
|
---|---|
Published in |
Memórias do Instituto Oswaldo Cruz, April 2015
|
DOI | 10.1590/0074-02760140375 |
Pubmed ID | |
Authors |
Liliane Maria Vidal Siqueira, Luciana Inácia Gomes, Edward Oliveira, Eduardo Ribeiro de Oliveira, Áureo Almeida de Oliveira, Martin Johannes Enk, Nídia Figueiredo Carneiro, Ana Rabello, Paulo Marcos Zech Coelho |
Abstract |
This study evaluated parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni. A population-based study was performed in 201 inhabitants from a low transmission locality named Pedra Preta, municipality of Montes Claros, state of Minas Gerais, Brazil. Four stool samples were analysed using two techniques, the Kato-Katz® (KK) technique (18 slides) and the TF-Test®, to establish the infection rate. The positivity rate of 18 KK slides of four stool samples was 28.9% (58/201) and the combined parasitological techniques (KK+TF-Test®) produced a 35.8% positivity rate (72/201). Furthermore, a polymerase chain reaction (PCR)-ELISA assay produced a positivity rate of 23.4% (47/201) using the first sample. All 72 patients with positive parasitological exams were treated with a single dose of Praziquantel® and these patients were followed-up 30, 90 and 180 days after treatment to establish the cure rate. Cure rates obtained by the analysis of 12 KK slides were 100%, 100% and 98.4% at 30, 90 and 180 days after treatment, respectively. PCR-ELISA revealed cure rates of 98.5%, 95.5% and 96.5%, respectively. The diagnostic and assessment of cure for schistosomiasis may require an increased number of KK slides or a test with higher sensitivity, such as PCR-ELISA, in situations of very low parasite load, such as after therapeutic interventions. |
Twitter Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 2% |
Unknown | 64 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 16 | 25% |
Student > Bachelor | 11 | 17% |
Researcher | 7 | 11% |
Student > Doctoral Student | 6 | 9% |
Student > Postgraduate | 5 | 8% |
Other | 9 | 14% |
Unknown | 11 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 15 | 23% |
Agricultural and Biological Sciences | 14 | 22% |
Biochemistry, Genetics and Molecular Biology | 9 | 14% |
Immunology and Microbiology | 4 | 6% |
Nursing and Health Professions | 3 | 5% |
Other | 8 | 12% |
Unknown | 12 | 18% |