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Characterization and expression analysis of WOX2 homeodomain transcription factor in Aegilops tauschii

Overview of attention for article published in Genetics and Molecular Biology, March 2015
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Title
Characterization and expression analysis of WOX2 homeodomain transcription factor in Aegilops tauschii
Published in
Genetics and Molecular Biology, March 2015
DOI 10.1590/s1415-475738138120140192
Pubmed ID
Authors

Shan Zhao, Qian-Tao Jiang, Jian Ma, Ji-Rui Wang, Ya-Xi Liu, Guo-Yue Chen, Peng-Fei Qi, Zhi-En Pu, Zhen-Xiang Lu, You-Liang Zheng, Yu-Ming Wei

Abstract

The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids. The predicted protein sequence contained a highly conserved homeodomain and the WUS-box domain, which is present in some members of the WOX protein family. The full-length ORF was subcloned into prokaryotic expression vector pET-30a, and an approximately 34-kDa protein was expressed in Escherichia coli BL21 (DE3) cells with IPTG induction. The molecular mass of the expressed protein was identical to that predicted by the cDNA sequence. Phylogenetic analysis suggested that Ae. tauschii WOX2 is closely related to the rice and maize orthologs. Quantitative PCR analysis showed that WOX2 from Ae. tauschii was primarily expressed in the seeds; transcription increased during seed development and declined after the embryos matured, suggesting that WOX2 is associated with embryo development in Ae. tauschii.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 16%
Other 2 11%
Lecturer 2 11%
Professor 2 11%
Researcher 2 11%
Other 2 11%
Unknown 6 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 32%
Agricultural and Biological Sciences 6 32%
Physics and Astronomy 1 5%
Unknown 6 32%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2015.
All research outputs
#3,598,742
of 5,124,332 outputs
Outputs from Genetics and Molecular Biology
#132
of 202 outputs
Outputs of similar age
#122,635
of 171,248 outputs
Outputs of similar age from Genetics and Molecular Biology
#10
of 15 outputs
Altmetric has tracked 5,124,332 research outputs across all sources so far. This one is in the 16th percentile – i.e., 16% of other outputs scored the same or lower than it.
So far Altmetric has tracked 202 research outputs from this source. They receive a mean Attention Score of 1.7. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
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We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.