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Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production

Overview of attention for article published in Microbial Cell Factories, April 2015
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  • Good Attention Score compared to outputs of the same age (69th percentile)

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1 tweeter
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2 patents

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81 Mendeley
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Title
Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production
Published in
Microbial Cell Factories, April 2015
DOI 10.1186/s12934-015-0246-0
Pubmed ID
Authors

Fotini A Trikka, Alexandros Nikolaidis, Anastasia Athanasakoglou, Aggeliki Andreadelli, Codruta Ignea, Konstantia Kotta, Anagnostis Argiriou, Sotirios C Kampranis, Antonios M Makris

Abstract

Terpenoids (isoprenoids) have numerous applications in flavors, fragrances, drugs and biofuels. The number of microbially produced terpenoids is increasing as new biosynthetic pathways are being elucidated. However, efforts to improve terpenoid production in yeast have mostly taken advantage of existing knowledge of the sterol biosynthetic pathway, while many additional factors may affect the output of the engineered system. Aiming to develop a yeast strain that can support high titers of sclareol, a diterpene of great importance for the perfume industry, we sought to identify gene deletions that improved carotenoid, and thus potentially sclareol, production. Using a carotenogenic screen, the best 100 deletion mutants, out of 4,700 mutant strains, were selected to create a subset for further analysis. To identify combinations of deletions that cooperate to further boost production, iterative carotenogenic screens were applied, and each time the top performing gene deletions were further ranked according to the number of genetic and physical interactions known for each specific gene. The gene selected in each round was deleted and the resulting strain was employed in a new round of selection. This approach led to the development of an EG60 derived haploid strain combining six deletions (rox1, dos2, yer134c, vba5, ynr063w and ygr259c) and exhibiting a 40-fold increase in carotenoid and 12-fold increase in sclareol titers, reaching 750 mg/L sclareol in shake flask cultivation. Using an iterative approach, we identified novel combinations of yeast gene deletions that improve carotenoid and sclareol production titers without compromising strain growth and viability. Most of the identified deletions have not previously been implicated in sterol pathway control. Applying the same approach using a different starting point could yield alternative sets of deletions with similar or improved outcome.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 1%
Serbia 1 1%
China 1 1%
Unknown 78 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 22 27%
Student > Ph. D. Student 15 19%
Student > Bachelor 8 10%
Student > Master 7 9%
Other 6 7%
Other 13 16%
Unknown 10 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 41 51%
Biochemistry, Genetics and Molecular Biology 14 17%
Chemistry 7 9%
Chemical Engineering 2 2%
Engineering 2 2%
Other 5 6%
Unknown 10 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2018.
All research outputs
#4,418,574
of 14,805,703 outputs
Outputs from Microbial Cell Factories
#269
of 1,071 outputs
Outputs of similar age
#70,246
of 232,862 outputs
Outputs of similar age from Microbial Cell Factories
#1
of 1 outputs
Altmetric has tracked 14,805,703 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 1,071 research outputs from this source. They receive a mean Attention Score of 3.7. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 232,862 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them