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Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation

Overview of attention for article published in Stem Cell Research & Therapy, April 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

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1 news outlet
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6 X users

Citations

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31 Dimensions

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65 Mendeley
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Title
Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation
Published in
Stem Cell Research & Therapy, April 2015
DOI 10.1186/s13287-015-0057-6
Pubmed ID
Authors

Andreja Brodarac, Tomo Šarić, Barbara Oberwallner, Shokoufeh Mahmoodzadeh, Klaus Neef, Julie Albrecht, Karsten Burkert, Matteo Oliverio, Filomain Nguemo, Yeong-Hoon Choi, Wolfram F Neiss, Ingo Morano, Jürgen Hescheler, Christof Stamm

Abstract

Induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) may be suitable for myocardial repair. While their functional and structural properties have been extensively investigated, their response to ischemia-like conditions has not yet been clearly defined. iPS-CMs were differentiated and enriched from murine iPS cells expressing eGFP and puromycin resistance genes under the control of an α-MHC promoter. iPS-CMs maturity and function were characterized by microscopy, rt-PCR, calcium transient recordings, electrophysiology, and mitochondrial function assays, and compared to those from neonatal murine cardiomyocytes (N-CMs). iPS-CMs as well as N-CMs were exposed for 3 h to hypoxia (1% O2) and glucose/serum deprivation (GSD), and viability, apoptosis markers, reactive oxygen species (ROS), mitochondrial membrane potential (Δψm) and intracellular stress signaling cascades were investigated. Then, the iPS-CMs response to mesenchymal stromal cell-conditioned medium (MSC-CoM) was determined. iPS-CMs displayed key morphological and functional properties that were comparable to those of N-CMs, but several parameters indicated an earlier iPS-CMs maturation stage. During hypoxia/GSD, iPS-CMs exhibited a significantly higher proportion of poly-caspase-active, 7-AAD- and TUNEL-positive cells than N-CMs. The average mitochondrial membrane potential (Δψm) was reduced in "ischemic" iPS-CMs but remained unchanged in N-CMs, ROS production was only increased in "ischemic" iPS-CMs, and oxidoreductase activity in iPS-CMs dropped more rapidly than in N-CMs. In iPS-CMs, hypoxia/GSD led to upregulation of Hsp70 transcripts and decreased STAT3 phosphorylation and total PKCε protein expression. Treatment with MSC-CoM preserved oxidoreductase activity and restored pSTAT3 and PKCε levels. iPS-CMs appear to be particularly sensitive to hypoxia and nutrient deprivation. Counteracting the ischemic susceptibility of iPS-CMs with MSC-conditioned medium may help enhance their survival and efficacy in cell-based approaches for myocardial repair.

X Demographics

X Demographics

The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 65 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 26%
Researcher 16 25%
Student > Master 13 20%
Student > Bachelor 6 9%
Student > Doctoral Student 5 8%
Other 3 5%
Unknown 5 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 31%
Biochemistry, Genetics and Molecular Biology 16 25%
Medicine and Dentistry 9 14%
Physics and Astronomy 3 5%
Engineering 3 5%
Other 8 12%
Unknown 6 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 May 2022.
All research outputs
#2,439,243
of 24,174,783 outputs
Outputs from Stem Cell Research & Therapy
#169
of 2,576 outputs
Outputs of similar age
#31,448
of 269,390 outputs
Outputs of similar age from Stem Cell Research & Therapy
#7
of 77 outputs
Altmetric has tracked 24,174,783 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,576 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,390 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.