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Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk

Overview of attention for article published in Brazilian Journal of Microbiology, June 2012
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  • Among the highest-scoring outputs from this source (#36 of 109)
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Citations

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27 Mendeley
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Title
Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk
Published in
Brazilian Journal of Microbiology, June 2012
DOI 10.1590/s1517-83822012000200015
Pubmed ID
Authors

Fernando Luiz Tobias, Luize Néli Nunes Garcia, Milton Masahiko Kanashiro, Enrique Medina-Acosta, João Gato Brom-de-Luna, Claudia Maria Costa de Almeida, Romildo Rocha Azevedo Junior, Môsar Lemos, Olney Vieira-da-Motta

Abstract

Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of sub products of ostrich raising, non fertilized eggs of four selected birds were utilized for development of polyclonal IgY antibodies. The birds were immunized (200ug/animal) with purified recombinant staphylococcal enterotoxin C (recSEC) and synthetic recRAP, both derived from S. aureus, and recBFPA and recEspB involved in E. coli pathogenicity, diluted in FCA injected in the braquial muscle. Two subsequent immunization steps with 21 days intervals were repeated in 0,85% saline in FIA. Blood and eggs samples were collected before and after immunization steps. Egg yolk immunoglobulins were purified by precipitation with 19% sodium sulfate and 20% ammonium sulphate methodologies. Purified IgY 50μL aliquots were incubated in 850μL BHI broth containing 50μL inoculums of five strains of S. aureus and five strains of E.coli during four hours at 37°C. Growth inhibition was evaluated followed by photometry reading (DO550nm). Egg yolk IgY preparation from hiperimmunized birds contained antibodies that inhibited significantly (p<0,05) growth of strains tested. Potential use of ostrich IgY polyclonal antibodies as a diagnostic and therapeutic tool is proposed for diseased animals.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 4%
Unknown 26 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 19%
Other 3 11%
Student > Postgraduate 2 7%
Student > Master 2 7%
Student > Ph. D. Student 2 7%
Other 3 11%
Unknown 10 37%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 4 15%
Immunology and Microbiology 4 15%
Agricultural and Biological Sciences 4 15%
Biochemistry, Genetics and Molecular Biology 4 15%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 3 11%
Unknown 7 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 April 2015.
All research outputs
#2,645,479
of 4,986,430 outputs
Outputs from Brazilian Journal of Microbiology
#36
of 109 outputs
Outputs of similar age
#96,549
of 168,250 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#1
of 1 outputs
Altmetric has tracked 4,986,430 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 109 research outputs from this source. They receive a mean Attention Score of 1.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 168,250 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them