| Chapter title |
Bioengineered skin humanized model of psoriasis.
|
|---|---|
| Chapter number | 20 |
| Book title |
Molecular Dermatology
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-227-8_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-226-1, 978-1-62703-227-8
|
| Authors |
Carretero M, Guerrero-Aspizua S, Del Río M, Marta Carretero, Sara Guerrero-Aspizua, Marcela Del Río, Carretero, Marta, Guerrero-Aspizua, Sara, Río, Marcela |
| Abstract |
This protocol describes the generation of a skin humanized mouse model for psoriasis using bioengineering approaches. This method is relatively simple, highly reproducible and ensures the obtention of a large and homogenous number of engrafted animals bearing a portion of human skin with psoriatic phenotype. The technique can employ cells from skin biopsies and blood samples from non-related healthy human donors (allogeneic version), as well as skin and blood cells from psoriatic patients (autologous version). In both cases, the psoriatic phenotype was developed after intradermal administration of in vitro derived T1 lymphocytes along with Th17 recombinant cytokines, in conjunction with mild barrier disruption by tape-stripping. This skin-humanized model for psoriasis emerges as a powerful tool to study the mechanisms underlying the pathogenesis of the disease. More importantly, the feasibility of the system may allow the evaluation of different therapeutic compounds in an in vivo system, employing local and/or systemic administration. |
X Demographics
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|---|---|---|
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Demographic breakdown
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| Members of the public | 2 | 100% |
Mendeley readers
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|---|---|---|
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| Unknown | 19 | 90% |
Demographic breakdown
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|---|---|---|
| Student > Ph. D. Student | 9 | 43% |
| Researcher | 3 | 14% |
| Librarian | 1 | 5% |
| Other | 1 | 5% |
| Professor | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 3 | 14% |
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| Immunology and Microbiology | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 3 | 14% |