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Isolation and development of microsatellite loci in an African Woodpecker (Campethera nivosa) using polymerase chain reaction and DNA sequencing

Overview of attention for article published in BMC Research Notes, May 2015
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Title
Isolation and development of microsatellite loci in an African Woodpecker (Campethera nivosa) using polymerase chain reaction and DNA sequencing
Published in
BMC Research Notes, May 2015
DOI 10.1186/s13104-015-1165-1
Pubmed ID
Authors

Nausheen F Khan, Kellie C Murdoch, Kevin A Feldheim, Ben D Marks, Norbert J Cordeiro

Abstract

The Buff-spotted Woodpecker (Campethera nivosa) is a resident bird species that is distributed in lowland rainforest habitats from western to eastern Africa. We developed species-specific microsatellite markers to examine the population genetics of this species. Twenty-one microsatellite loci were isolated from C. nivosa. Of these, 15 were found to amplify consistently. These loci were then tested for variability in 15 individuals from different lowland forest localities. The number of alleles ranged from 3 to 13 per locus, with observed and expected heterozygosity ranging from 0.100 to 0.917 and 0.485 to 0.901, respectively. Four loci exhibited significant heterozygote deficiency while one had an excess of heterozygotes. None of the loci exhibited linkage disequilibrium. These polymorphic microsatellite markers will be used to study genetic variability in populations of C. nivosa across either sides of the Congo River to evaluate the effect of the river as a barrier to gene flow.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 40%
Student > Postgraduate 1 20%
Student > Doctoral Student 1 20%
Unspecified 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 60%
Biochemistry, Genetics and Molecular Biology 1 20%
Unspecified 1 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2015.
All research outputs
#3,633,867
of 5,173,976 outputs
Outputs from BMC Research Notes
#1,113
of 1,641 outputs
Outputs of similar age
#124,132
of 174,324 outputs
Outputs of similar age from BMC Research Notes
#52
of 74 outputs
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