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Multiple-locus variable-number tandem-repeat analysis (MLVA) genotyping of human Brucella isolates in Malaysia

Overview of attention for article published in BMC Infectious Diseases, June 2015
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Title
Multiple-locus variable-number tandem-repeat analysis (MLVA) genotyping of human Brucella isolates in Malaysia
Published in
BMC Infectious Diseases, June 2015
DOI 10.1186/s12879-015-0958-0
Pubmed ID
Authors

Bee Yong Tay, Norazah Ahmad, Rohaidah Hashim, Jama’ayah Mohamed Zahidi, Kwai Lin Thong, Xiu Pei Koh, Azura Mohd Noor

Abstract

Brucellosis is one of the most common zoonotic diseases worldwide. It can cause acute febrile illness in human and is a major health problem. Studies in human brucellosis in Malaysia is limited and so far no genotyping studies has been done on Brucella isolates. The aim of the study was to determine the genetic diversity among Brucella species isolated from human brucellosis, obtained over a 6-year period (2009-2014). In this study, the genotypic characteristics of 43 human Brucella melitensis isolates were analysed using multiple-locus variable-number tandem-repeat analysis (MLVA) which consisted of eight minisatellite loci (panel 1) and eight microsatellite loci; panels 2A (3 microsatellite loci) and panel 2B (5 microsatellite loci). Two human Brucella suis isolates were also investigated using the MLVA assay. Using panel 1 (MLVA8), two genotypes namely genotype 43 and 44 were obtained from the 43 B. melitensis isolates. Using the combination of panels 1 and 2A loci (MLVA11), two genotypes were obtained while using the complete panels 1, 2A and 2B, nine genotypes were obtained. The polymorphisms in using the complete panels (MLVA16) were observed in three loci from panel 2B, which showed a diversity index higher than 0.17. All B. melitensis isolates were closely related to the East Mediterranean group. For B. suis isolates, only genotype 6 and genotype 33 were obtained using panel 1 and MLVA11 respectively. In conclusion, the results of the present study showed a low genetic diversity among B. melitensis and B. suis isolates from human patients. Based on the MLVA16 assay, B. melitensis belonging to the East Mediterranean group is responsible for the vast majority of Brucella infections in our Malaysian patients. To our knowledge, this is the first genotyping study of human Brucella isolates in Malaysia.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 6%
Unknown 16 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 24%
Student > Bachelor 2 12%
Student > Doctoral Student 2 12%
Student > Master 2 12%
Student > Ph. D. Student 2 12%
Other 5 29%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 4 24%
Medicine and Dentistry 4 24%
Unspecified 3 18%
Immunology and Microbiology 3 18%
Agricultural and Biological Sciences 2 12%
Other 1 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2015.
All research outputs
#3,639,201
of 5,180,561 outputs
Outputs from BMC Infectious Diseases
#2,043
of 2,726 outputs
Outputs of similar age
#124,345
of 174,946 outputs
Outputs of similar age from BMC Infectious Diseases
#70
of 98 outputs
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