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Draft genomes of Shigella strains used by the STOPENTERICS consortium

Overview of attention for article published in Gut Pathogens, June 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • Good Attention Score compared to outputs of the same age and source (77th percentile)

Mentioned by

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4 tweeters

Citations

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4 Dimensions

Readers on

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8 Mendeley
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2 CiteULike
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Title
Draft genomes of Shigella strains used by the STOPENTERICS consortium
Published in
Gut Pathogens, June 2015
DOI 10.1186/s13099-015-0061-5
Pubmed ID
Authors

Omar Rossi, Kate S Baker, Armelle Phalipon, François-Xavier Weill, Francesco Citiulo, Philippe Sansonetti, Christiane Gerke, Nicholas R Thomson

Abstract

Despite a significant global burden of disease, there is still no vaccine against shigellosis widely available. One aim of the European Union funded STOPENTERICS consortium is to develop vaccine candidates against Shigella. Given the importance of translational vaccine coverage, here we aimed to characterise the Shigella strains being used by the consortium by whole genome sequencing, and report on the stability of strains cultured in different laboratories or through serial passage. We sequenced, de novo assembled and annotated 20 Shigella strains being used by the consortium. These comprised 16 different isolates belonging to 7 serotypes, and 4 derivative strains. Derivative strains from common isolates were manipulated in different laboratories or had undergone multiple passages in the same laboratory. Strains were mapped against reference genomes to detect SNP variation and phylogenetic analysis was performed. The genomes assembled into similar total lengths (range 4.14-4.83 Mbp) and had similar numbers of predicted coding sequences (average of 4,400). Mapping analysis showed the genetic stability of strains through serial passages and culturing in different laboratories, as well as varying levels of similarity to published reference genomes. Phylogenetic analysis revealed the presence of three main clades among the strains and published references, one containing the Shigella flexneri serotype 6 strains, a second containing the remaining S. flexneri serotypes and a third comprised of Shigella sonnei strains. This work increases the number of the publically available Shigella genomes available and specifically provides information on strains being used for vaccine development by STOPENTERICS. It also provides information on the variability among strains maintained in different laboratories and through serial passage. This work will guide the selection of strains for further vaccine development.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 25%
Researcher 2 25%
Student > Doctoral Student 1 13%
Student > Ph. D. Student 1 13%
Unknown 2 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 25%
Immunology and Microbiology 1 13%
Computer Science 1 13%
Physics and Astronomy 1 13%
Medicine and Dentistry 1 13%
Other 0 0%
Unknown 2 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 June 2015.
All research outputs
#2,866,921
of 7,435,531 outputs
Outputs from Gut Pathogens
#65
of 195 outputs
Outputs of similar age
#74,625
of 216,876 outputs
Outputs of similar age from Gut Pathogens
#2
of 9 outputs
Altmetric has tracked 7,435,531 research outputs across all sources so far. This one has received more attention than most of these and is in the 61st percentile.
So far Altmetric has tracked 195 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 216,876 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 7 of them.