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Expression and functional characterisation of System L amino acid transporters in the human term placenta

Overview of attention for article published in Reproductive Biology and Endocrinology, June 2015
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  • Good Attention Score compared to outputs of the same age (74th percentile)

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Title
Expression and functional characterisation of System L amino acid transporters in the human term placenta
Published in
Reproductive Biology and Endocrinology, June 2015
DOI 10.1186/s12958-015-0054-8
Pubmed ID
Authors

Francesca Gaccioli, Irving L. M. H. Aye, Sara Roos, Susanne Lager, Vanessa I. Ramirez, Yoshikatsu Kanai, Theresa L. Powell, Thomas Jansson

Abstract

System L transporters LAT1 (SLC7A5) and LAT2 (SLC7A8) mediate the uptake of large, neutral amino acids in the human placenta. Many System L substrates are essential amino acids, thus representing crucial nutrients for the growing fetus. Both LAT isoforms are expressed in the human placenta, but the relative contribution of LAT1 and LAT2 to placental System L transport and their subcellular localisation are not well established. Moreover, the influence of maternal body mass index (BMI) on placental System L amino acid transport is poorly understood. Therefore the aims of this study were to determine: i) the relative contribution of the LAT isoforms to System L transport activity in primary human trophoblast (PHT) cells isolated from term placenta; ii) the subcellular localisation of LAT transporters in human placenta; and iii) placental expression and activity of System L transporters in response to maternal overweight/obesity. System L mediated leucine uptake was measured in PHT cells after treatment with si-RNA targeting LAT1 and/or LAT2. The localisation of LAT isoforms was studied in isolated microvillous plasma membranes (MVM) and basal membranes (BM) by Western blot analysis. Results were confirmed by immunohistochemistry in sections of human term placenta. Expression and activity System L transporters was measured in isolated MVM from women with varying pre-pregnancy BMI. Both LAT1 and LAT2 isoforms contribute to System L transport activity in primary trophoblast cells from human term placenta. LAT1 and LAT2 transporters are highly expressed in the MVM of the syncytiotrophoblast layer at term. LAT2 is also localised in the basal membrane and in endothelial cells lining the fetal capillaries. Measurements in isolated MVM vesicles indicate that System L transporter expression and activity is not influenced by maternal BMI. LAT1 and LAT2 are present and functional in the syncytiotrophoblast MVM, whereas LAT2 is also expressed in the BM and in the fetal capillary endothelium. In contrast to placental System A and beta amino acid transporters, MVM System L activity is unaffected by maternal overweight/obesity.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Czechia 1 2%
Brazil 1 2%
Unknown 49 94%

Demographic breakdown

Readers by professional status Count As %
Student > Master 13 25%
Student > Bachelor 9 17%
Student > Ph. D. Student 8 15%
Student > Doctoral Student 6 12%
Researcher 4 8%
Other 9 17%
Unknown 3 6%
Readers by discipline Count As %
Medicine and Dentistry 17 33%
Biochemistry, Genetics and Molecular Biology 12 23%
Agricultural and Biological Sciences 11 21%
Pharmacology, Toxicology and Pharmaceutical Science 5 10%
Nursing and Health Professions 2 4%
Other 1 2%
Unknown 4 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2019.
All research outputs
#3,456,811
of 13,532,333 outputs
Outputs from Reproductive Biology and Endocrinology
#135
of 541 outputs
Outputs of similar age
#59,495
of 234,677 outputs
Outputs of similar age from Reproductive Biology and Endocrinology
#1
of 1 outputs
Altmetric has tracked 13,532,333 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 541 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 234,677 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them