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Molecular Mechanisms Involved in Vascular Interactions of the Lyme Disease Pathogen in a Living Host

Overview of attention for article published in PLoS Pathogens, October 2008
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  • Good Attention Score compared to outputs of the same age (69th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

4 tweeters
2 Facebook pages


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Readers on

45 Mendeley
1 CiteULike
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Molecular Mechanisms Involved in Vascular Interactions of the Lyme Disease Pathogen in a Living Host
Published in
PLoS Pathogens, October 2008
DOI 10.1371/journal.ppat.1000169
Pubmed ID

M. Ursula Norman, Tara J. Moriarty, Ashley R. Dresser, Brandie Millen, Paul Kubes, George Chaconas


Hematogenous dissemination is important for infection by many bacterial pathogens, but is poorly understood because of the inability to directly observe this process in living hosts at the single cell level. All disseminating pathogens must tether to the host endothelium despite significant shear forces caused by blood flow. However, the molecules that mediate tethering interactions have not been identified for any bacterial pathogen except E. coli, which tethers to host cells via a specialized pillus structure that is not found in many pathogens. Furthermore, the mechanisms underlying tethering have never been examined in living hosts. We recently engineered a fluorescent strain of Borrelia burgdorferi, the Lyme disease pathogen, and visualized its dissemination from the microvasculature of living mice using intravital microscopy. We found that dissemination was a multistage process that included tethering, dragging, stationary adhesion and extravasation. In the study described here, we used quantitative real-time intravital microscopy to investigate the mechanistic features of the vascular interaction stage of B. burgdorferi dissemination. We found that tethering and dragging interactions were mechanistically distinct from stationary adhesion, and constituted the rate-limiting initiation step of microvascular interactions. Surprisingly, initiation was mediated by host Fn and GAGs, and the Fn- and GAG-interacting B. burgdorferi protein BBK32. Initiation was also strongly inhibited by the low molecular weight clinical heparin dalteparin. These findings indicate that the initiation of spirochete microvascular interactions is dependent on host ligands known to interact in vitro with numerous other bacterial pathogens. This conclusion raises the intriguing possibility that fibronectin and GAG interactions might be a general feature of hematogenous dissemination by other pathogens.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Netherlands 1 2%
Sweden 1 2%
Iran, Islamic Republic of 1 2%
Mexico 1 2%
Unknown 40 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 24%
Researcher 9 20%
Student > Bachelor 7 16%
Professor > Associate Professor 5 11%
Professor 4 9%
Other 7 16%
Unknown 2 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 44%
Medicine and Dentistry 9 20%
Biochemistry, Genetics and Molecular Biology 6 13%
Immunology and Microbiology 6 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 2 4%
Unknown 1 2%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2019.
All research outputs
of 14,150,306 outputs
Outputs from PLoS Pathogens
of 6,420 outputs
Outputs of similar age
of 109,175 outputs
Outputs of similar age from PLoS Pathogens
of 129 outputs
Altmetric has tracked 14,150,306 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 6,420 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.5. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 109,175 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 129 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.