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Intracellular trafficking of factor VIII to von Willebrand factor storage granules.

Overview of attention for article published in Journal of Clinical Investigation, February 1998
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (72nd percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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3 patents

Citations

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82 Dimensions

Readers on

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24 Mendeley
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Title
Intracellular trafficking of factor VIII to von Willebrand factor storage granules.
Published in
Journal of Clinical Investigation, February 1998
DOI 10.1172/jci1250
Pubmed ID
Authors

J B Rosenberg, P A Foster, R J Kaufman, E A Vokac, M Moussalli, P A Kroner, R R Montgomery

Abstract

In plasma, von Willebrand factor (vWf) associates with Factor VIII (FVIII); however, the site at which these proteins first interact has not been defined. Administration of 1-desamino-8-D-arginine vasopressin (DDAVP) causes a rapid, concomitant elevation in plasma levels of both vWf and FVIII, suggesting the existence of a DDAVP-releasable storage pool for both proteins. To determine whether vWf and FVIII can associate intracellularly and colocalize to storage vesicles, we transfected AtT-20 cells with vWf and FVIII expression plasmids. FVIII alone was not detectable within storage granules; however, transfection of vWf cDNA into the same cell caused FVIII to alter its intracellular trafficking and to undergo granular storage, colocalizing to the vWf-containing granules. In contrast, colocalization of FVIII was not observed when these cells were transfected with plasmids encoding defective FVIII-binding vWf mutants. Transfection of bovine endothelial cells with FVIII further demonstrated vesicular storage of FVIII with vWf in Weibel-Palade bodies. Since gene therapy of hemophilia A may ultimately target endothelium or hematopoietic stem cells, the interaction between vWf and FVIII within a secretory cell is important. Thus, vWf can alter the intracellular trafficking of FVIII from a constitutive to a regulated secretory pathway, thereby producing an intracellular storage pool of both proteins.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 2 8%
Germany 1 4%
United States 1 4%
Unknown 20 83%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 50%
Student > Master 5 21%
Professor 2 8%
Student > Ph. D. Student 1 4%
Student > Doctoral Student 1 4%
Other 3 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 33%
Medicine and Dentistry 7 29%
Immunology and Microbiology 3 13%
Biochemistry, Genetics and Molecular Biology 2 8%
Psychology 1 4%
Other 1 4%
Unknown 2 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 May 2019.
All research outputs
#2,894,435
of 15,071,208 outputs
Outputs from Journal of Clinical Investigation
#4,352
of 14,105 outputs
Outputs of similar age
#75,899
of 277,226 outputs
Outputs of similar age from Journal of Clinical Investigation
#73
of 120 outputs
Altmetric has tracked 15,071,208 research outputs across all sources so far. Compared to these this one has done well and is in the 77th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 14,105 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 12.1. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,226 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 120 others from the same source and published within six weeks on either side of this one. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.