Title |
Amperometric determination of As(III) and Cd(II) using a platinum electrode modified with acetylcholinesterase, ruthenium(II)-tris(bipyridine) and graphene oxide
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Published in |
Microchimica Acta, May 2018
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DOI | 10.1007/s00604-018-2822-6 |
Pubmed ID | |
Authors |
Manju Bhargavi Gumpu, Murugan Veerapandian, Uma Maheswari Krishnan, John Bosco Balaguru Rayappan |
Abstract |
The authors describe an amperometric biosensor for the determination As(III) and Cd(II) based on the inhibition of the enzyme acetylcholineesterase (AChE). A platinum electrode was modified with ruthenium(II)-tris(bipyridyl), graphene oxide and AChE and then showed redox peaks at 0.06 and 0.2 V vs Ag/AgCl in the presence of acetylthiocholine chloride (ATChCl). Amperometry unveiled a steady-state turnover rate with the release of thiocholine. In the presence of arsenic(III) and cadmium(II), AChE showed an inhibitive response at 0.214 and 0.233 V vs Ag/AgCl, respectively. The electrode exhibits a detection limit and linear range of 0.03 μM and 0.05-0.8 μM for As(III) and 0.07 μM and 0.02-0.7 μM for Cd(II), respectively. Type of inhibition and inhibition constants induced by As(III) and Cd(II) on the catalytic sites of AChE were determined from Dixon and Lineweaver-Burk plots. The modified electrode was applied to the determination of As3+ and Cd2+ in river, tap and waste water, and the results proved that the method is sensitive and can be an alternative to chromatographic and spectroscopic techniques. Graphical abstract Schematic presentation of Pt/Ru(II)-tris(bipy)-GO/AChE electrode in absence and presence of metal ions (As3+/Cd2+). |
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