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N 6 -methyl-adenosine level in Nicotiana tabacum is associated with tobacco mosaic virus

Overview of attention for article published in Virology Journal, May 2018
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26 Mendeley
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Title
N 6 -methyl-adenosine level in Nicotiana tabacum is associated with tobacco mosaic virus
Published in
Virology Journal, May 2018
DOI 10.1186/s12985-018-0997-4
Pubmed ID
Authors

Zhurui Li, Jing Shi, Lu Yu, Xiaozhen Zhao, Longlu Ran, Deyu Hu, Baoan Song

Abstract

N 6 -methyl-adenosine (m6A) is a prevalent RNA modification in many species. Abnormal m6A methylation levels can lead to RNA dysfunction and can cause diseases. Tobacco mosaic virus (TMV) is one of the most devastating viruses for agricultural plants. It has many hosts, particularly including tobacco and other members the family Solanaceae. However, it remains unclear whether the abnormal growth induced by TMV is associated with the m6A level. A rapid and accurate analytical method using ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HR - MS/MS) was developed to analyse the adenosine (A), cytidine (C), guanosine (G), uridine (U), and m6A contents in the tobacco leaf, and the m6A/G ratio was used to evaluate the m6A level. Subsequent protein sequence alignments were used to find the potential methylases and demethylases in Nicotiana tabacum (N. tabacum). Finally, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was used to analyse the gene expression levels of the potential methylases and demethylases in the N. tabacum leaf. The results showed that TMV reduced the m6A level. Moreover, protein sequence alignments revealed partial homology among human ALKBH5, Arabidopsis (NP_001031793), and Nicotiana sylvestris (XP_009800010). The gene expression level of the potential demethylase XM_009801708 increased at 14 and 21 days in N. tabacum infected with TMV, whereas all of the potential methylases decreased. The reversible m6A modification in N. tabacum mRNA might represent a novel epigenetic mechanism involved in TMV.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 31%
Student > Master 3 12%
Student > Bachelor 2 8%
Researcher 2 8%
Professor 1 4%
Other 1 4%
Unknown 9 35%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 35%
Agricultural and Biological Sciences 7 27%
Chemistry 1 4%
Unknown 9 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2018.
All research outputs
#7,186,031
of 12,959,872 outputs
Outputs from Virology Journal
#929
of 2,143 outputs
Outputs of similar age
#129,171
of 271,106 outputs
Outputs of similar age from Virology Journal
#1
of 1 outputs
Altmetric has tracked 12,959,872 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,143 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,106 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them