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Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae

Overview of attention for article published in Parasites & Vectors, May 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (68th percentile)

Mentioned by

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7 tweeters

Citations

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3 Dimensions

Readers on

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18 Mendeley
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Title
Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae
Published in
Parasites & Vectors, May 2018
DOI 10.1186/s13071-018-2893-6
Pubmed ID
Authors

Vera T. Unwin, Shaun Ainsworth, Emily J. Rippon, El Hadji Amadou Niang, Mark J. I. Paine, David Weetman, Emily R. Adams

Abstract

The spread of insecticide resistance (IR) is a major threat to vector control programmes for mosquito-borne diseases. Early detection of IR using diagnostic markers could help inform these programmes, especially in remote locations where gathering reliable bioassay data is challenging. Most current molecular tests for genetic IR markers are only suitable for use in well-equipped laboratory settings. There is an unmet need for field-applicable diagnostics. A single-cartridge test was designed to detect key IR mutations in the major African vector of malaria, Anopheles gambiae. Developed on the portable, rapid, point-of-care compatible PCR platform - Genedrive® (genedrive® plc), the test comprises two assays which target single nucleotide polymorphisms (SNPs) in the voltage gated sodium channel (VGSC) gene that exert interactive effects on knockdown resistance (kdr): L1014F, L1014S and N1575Y. Distinct melt peaks were observed for each allele at each locus. Preliminary validation of these assays using a test panel of 70 An. gambiae samples showed complete agreement of our assays with the widely-used TaqMan assays, achieving a sensitivity and specificity of 100%. Here we show the development of an insecticide resistance detection assay for use on the Genedrive® platform that has the potential to be the first field-applicable diagnostic for kdr.

Twitter Demographics

The data shown below were collected from the profiles of 7 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 28%
Researcher 4 22%
Professor 2 11%
Student > Bachelor 2 11%
Student > Master 1 6%
Other 0 0%
Unknown 4 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 28%
Biochemistry, Genetics and Molecular Biology 4 22%
Environmental Science 1 6%
Immunology and Microbiology 1 6%
Physics and Astronomy 1 6%
Other 1 6%
Unknown 5 28%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2018.
All research outputs
#3,347,348
of 13,528,132 outputs
Outputs from Parasites & Vectors
#694
of 3,605 outputs
Outputs of similar age
#83,947
of 268,949 outputs
Outputs of similar age from Parasites & Vectors
#1
of 2 outputs
Altmetric has tracked 13,528,132 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,605 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,949 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them