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Sequence analysis of DNA randomly amplified from theSaccharomyces cerevisiaegenome

Overview of attention for article published in Molecular & Cellular Probes, December 1998
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)

Mentioned by

patent
18 patents

Citations

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15 Dimensions

Readers on

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7 Mendeley
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Title
Sequence analysis of DNA randomly amplified from theSaccharomyces cerevisiaegenome
Published in
Molecular & Cellular Probes, December 1998
DOI 10.1006/mcpr.1998.0199
Pubmed ID
Authors

A McGrath, D.G Higgins, T.V McCarthy

Abstract

Despite its widespread use, the molecular basis of random amplification is poorly understood. Here the basis of random amplification has been investigated by cloning and sequencing the products of a random amplification of polymorphic DNA (RAPD) amplification from Saccharomyces cerevisiae DNA. The genomic origin of the amplified products was determined by sequence comparison with the S. cerevisiae Genome Database (SGD). This allowed analysis of the degree of identity between the random primer and the primer binding sites on the genome. There was no relationship between RAPD size, GC content and relative abundance. The degree of matching between the primer and the primer binding sites increased towards the 3; end of the primer and decreased towards the 5; end. The maximum number of mismatches observed between primer and primer binding sites was never more than one between positions 1-7 of the primer. Nucleotide compositional biases were also observed upstream and downstream of the primer binding site with a marked preference for AT richness upstream of the primer binding sites and for a GC preference directly following the 3; end of the primer. These findings have important ramifications for primer design for multiplex, low stringency and degenerate polymerase chain reaction (PCR).

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 14%
Unknown 6 86%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 43%
Student > Postgraduate 1 14%
Student > Doctoral Student 1 14%
Student > Master 1 14%
Unknown 1 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 86%
Unknown 1 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2022.
All research outputs
#4,619,572
of 22,479,668 outputs
Outputs from Molecular & Cellular Probes
#83
of 819 outputs
Outputs of similar age
#9,477
of 102,100 outputs
Outputs of similar age from Molecular & Cellular Probes
#1
of 3 outputs
Altmetric has tracked 22,479,668 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 819 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 102,100 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them