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Molecular epidemiology and characterization of an outbreak causing Klebsiella pneumoniae clone carrying chromosomally located bla CTX-M-15 at a German University-Hospital

Overview of attention for article published in BMC Microbiology, June 2015
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Title
Molecular epidemiology and characterization of an outbreak causing Klebsiella pneumoniae clone carrying chromosomally located bla CTX-M-15 at a German University-Hospital
Published in
BMC Microbiology, June 2015
DOI 10.1186/s12866-015-0460-2
Pubmed ID
Authors

Stephen E. Mshana, Moritz Fritzenwanker, Linda Falgenhauer, Eugen Domann, Torsten Hain, Trinad Chakraborty, Can Imirzalioglu

Abstract

Multi-drug resistant Klebsiella pneumoniae strains are a common cause of health care associated infections worldwide. Clonal spread of Klebsiella pneumoniae isolates carrying plasmid mediated CTX-M-15 have been commonly reported. Limited data is available regarding dissemination of chromosomally encoded CTX-M-15 in Klebsiella pneumoniae worldwide. We examined 23 non-repetitive ESBL-producing Klebsiella pneumoniae strains isolated from clinical specimens over a period of 4 months in a German University Hospital. All isolates were characterized to determine their genetic relatedness using Pulsed-Field Gel Electrophoresis (PFGE) and Multi Locus Sequence Typing (MLST). PFGE revealed three clusters (B1, B2, and B3) with a sub-cluster (A3) comprising of 10 isolates with an identical PFGE pattern. All strains of the cluster B3 with similar PFGE patterns were typed as ST101, indicating an outbreak situation. The ESBL allele bla CTX-M-15 was identified in 16 (69.6 %) of all isolates, including all of the outbreak strains. Within the A3 sub-cluster, the CTX-M-15 allele could not be transferred by conjugation. DNA hybridization studies suggested a chromosomal location of bla CTX-M-15. Whole genome sequencing located CTX-M-15 within a complete ISEcp-1 transposition unit inserted into an ORF encoding for a putative membrane protein. PCR-based analysis of the flanking regions demonstrated that insertion into this region is unique and present in all outbreak isolates. This is the first characterization of a chromosomal insertion of bla CTX-M-15 in Klebsiella pneumonia ST101, a finding suggesting that in Enterobacteriaceae, chromosomal locations may also act as reservoirs for the spread of bla CTX-M-15 encoding transposition units.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Unknown 51 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 19%
Student > Master 10 19%
Researcher 9 17%
Student > Doctoral Student 4 8%
Student > Postgraduate 2 4%
Other 5 10%
Unknown 12 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 21%
Medicine and Dentistry 8 15%
Immunology and Microbiology 6 12%
Biochemistry, Genetics and Molecular Biology 5 10%
Arts and Humanities 1 2%
Other 4 8%
Unknown 17 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2016.
All research outputs
#6,293,846
of 8,294,943 outputs
Outputs from BMC Microbiology
#937
of 1,378 outputs
Outputs of similar age
#159,389
of 229,420 outputs
Outputs of similar age from BMC Microbiology
#30
of 45 outputs
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