↓ Skip to main content

Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, May 2018
Altmetric Badge

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
7 Dimensions

Readers on

mendeley
50 Mendeley
Title
Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking
Published in
Proceedings of the National Academy of Sciences of the United States of America, May 2018
DOI 10.1073/pnas.1804091115
Pubmed ID
Authors

Lauren V. Albrecht, Diego Ploper, Nydia Tejeda-Muñoz, Edward M. De Robertis

Abstract

Arginine methylation has emerged as a widespread and reversible protein modification with the potential to regulate a multitude of cellular processes, but its function is poorly understood. Endolysosomes play an important role in Wnt signaling, in which glycogen synthase kinase 3 (GSK3) becomes sequestered inside multivesicular bodies (MVBs) by the process known as microautophagy, causing the stabilization of many proteins. Up to 20% of cellular proteins contain three or more consecutive putative GSK3 sites, and of these 33% also contain methylarginine (meArg) modifications. Intriguingly, a cytoskeletal protein was previously known to have meArg modifications that enhanced subsequent phosphorylations by GSK3. Here, we report the unexpected finding that protein arginine methyltransferase 1 (PRMT1) is required for canonical Wnt signaling. Treatment of cultured cells for 5-30 min with Wnt3a induced a large increase in total endocytic vesicles which were also positive for asymmetric dimethylarginine modifications. Protease protection studies, both biochemical and in situ in cultured cells, showed that many meArg-modified cytosolic proteins became rapidly translocated into MVBs together with GSK3 and Lys48-polyubiquitinated proteins by ESCRT-driven microautophagy. In the case of the transcription factor Smad4, we showed that a unique arginine methylation site was required for GSK3 phosphorylation and Wnt regulation. The enzyme PRMT1 was found to be essential for Wnt-stimulated arginine methylation, GSK3 sequestration, and canonical Wnt signaling. The results reveal a cell biological role for PRMT1 arginine methylation at the crossroads of growth factor signaling, protein phosphorylation, membrane trafficking, cytosolic proteolysis, and Wnt-regulated microautophagy.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 50 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 38%
Student > Bachelor 8 16%
Researcher 6 12%
Student > Master 5 10%
Librarian 1 2%
Other 5 10%
Unknown 6 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 25 50%
Agricultural and Biological Sciences 9 18%
Medicine and Dentistry 3 6%
Immunology and Microbiology 2 4%
Chemical Engineering 1 2%
Other 4 8%
Unknown 6 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 May 2018.
All research outputs
#9,939,072
of 12,988,455 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#74,126
of 79,132 outputs
Outputs of similar age
#186,744
of 269,919 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#868
of 992 outputs
Altmetric has tracked 12,988,455 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 79,132 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 23.2. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,919 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 992 others from the same source and published within six weeks on either side of this one. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.