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Isolation and biochemical characterization of a thaumatin-like kiwi allergen

Overview of attention for article published in The Journal of Allergy and Clinical Immunology, November 2002
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  • Above-average Attention Score compared to outputs of the same age (58th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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5 patents

Citations

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98 Dimensions

Readers on

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53 Mendeley
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Title
Isolation and biochemical characterization of a thaumatin-like kiwi allergen
Published in
The Journal of Allergy and Clinical Immunology, November 2002
DOI 10.1067/mai.2002.128947
Pubmed ID
Authors

Marija Gavrović-Jankulović, Tanja Ćirković, Olga Vučković, Marina Atanasković-Marković, Arnd Petersen, Gordana Gojgić, Lidija Burazer, Ratko M. Jankov

Abstract

Kiwi fruit allergy, as well as its association with hypersensitivity to other foods and to pollen, has been extensively reported in the last few years. Several IgE-binding components have been detected in kiwi extract, but only one 30- kd allergen has been isolated; it was identified as actinidin (Act c 1). Recently, we have reported a 24-kd kiwi protein to be a potential major allergen in a group of patients with oral allergy syndrome (OAS). The aim of this study was to purify and characterize the 24-kd kiwi allergen biochemically. Seven polysensitized patients with OAS to kiwi were used in this study. The kiwi allergen was isolated by using a combination of gel permeation, ion exchange, and immobilized metal ion affinity chromatography. Its biochemical characterization included determination of its isoelectric point, molecular weight, N-terminal sequencing, concanavalin A -binding ability, digestibility in simulated gastric fluid, and antifungal activity. Western blotting, 2-dimensional PAGE immunoblotting, and skin prick tests were performed to characterize the isolated protein immunochemically. All 7 patients recognized the isolated 24-kd kiwi protein as an allergen. The isolated protein consisted of 2 isoforms with isoelectric points of 9.4 and 9.5 migrated as one protein band of 20 kd after SDS-PAGE under nonreducing conditions or at 24 kd under reducing conditions. The partial N-terminal sequence revealed that it is a thaumatin-like protein (TLP) with concanavalin A -binding ability. The protein showed antifungal activity toward Saccharomyces carlsbergensis, and Candida albicans. The protein was degraded by the simulated gastric fluid within 1 minute. Both isoforms bound IgE from a pool of sera in a 2-dimensional PAGE immunoblot. The TLP elicited positive skin prick test responses in 4 (80 %) of 5 patients with OAS. This study reported isolation and full characterization of a new kiwi allergen, TLP (isoelectric points of 9.4 and 9.5 and molecular weight of 24 kd), which belongs to the family of pathogenesis-related proteins. The isolated protein expressed antifungal activity toward S carlsbergensis and C albicans.

Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Mexico 1 2%
Unknown 51 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 21%
Student > Ph. D. Student 11 21%
Student > Doctoral Student 6 11%
Student > Bachelor 5 9%
Professor 3 6%
Other 6 11%
Unknown 11 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 28%
Medicine and Dentistry 6 11%
Immunology and Microbiology 5 9%
Biochemistry, Genetics and Molecular Biology 4 8%
Chemistry 4 8%
Other 8 15%
Unknown 11 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2014.
All research outputs
#5,610,509
of 17,368,632 outputs
Outputs from The Journal of Allergy and Clinical Immunology
#4,817
of 9,282 outputs
Outputs of similar age
#111,647
of 287,949 outputs
Outputs of similar age from The Journal of Allergy and Clinical Immunology
#77
of 138 outputs
Altmetric has tracked 17,368,632 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,282 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.2. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 287,949 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 138 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.