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Attention Score in Context
| Title |
Precise control of SCRaMbLE in synthetic haploid and diploid yeast
|
|---|---|
| Published in |
Nature Communications, May 2018
|
| DOI | 10.1038/s41467-018-03084-4 |
| Pubmed ID | |
| Authors |
Bin Jia, Yi Wu, Bing-Zhi Li, Leslie A. Mitchell, Hong Liu, Shuo Pan, Juan Wang, Hao-Ran Zhang, Nan Jia, Bo Li, Michael Shen, Ze-Xiong Xie, Duo Liu, Ying-Xiu Cao, Xia Li, Xiao Zhou, Hao Qi, Jef D. Boeke, Ying-Jin Yuan |
| Abstract |
Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast cells incorporating the Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system provide a platform for generating genotype diversity. Here we construct a genetic AND gate to enable precise control of the SCRaMbLE method to generate synthetic haploid and diploid yeast with desired phenotypes. The yield of carotenoids is increased to 1.5-fold by SCRaMbLEing haploid strains and we determine that the deletion of YEL013W is responsible for the increase. Based on the SCRaMbLEing in diploid strains, we develop a strategy called Multiplex SCRaMbLE Iterative Cycling (MuSIC) to increase the production of carotenoids up to 38.8-fold through 5 iterative cycles of SCRaMbLE. This strategy is potentially a powerful tool for increasing the production of bio-based chemicals and for mining deep knowledge. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 123 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 123 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 23 | 19% |
| Student > Master | 20 | 16% |
| Student > Ph. D. Student | 16 | 13% |
| Researcher | 14 | 11% |
| Student > Doctoral Student | 6 | 5% |
| Other | 10 | 8% |
| Unknown | 34 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 44 | 36% |
| Agricultural and Biological Sciences | 20 | 16% |
| Engineering | 4 | 3% |
| Chemical Engineering | 4 | 3% |
| Chemistry | 4 | 3% |
| Other | 8 | 7% |
| Unknown | 39 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 41. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 May 2018.
All research outputs
#983,209
of 24,940,046 outputs
Outputs from Nature Communications
#15,922
of 54,625 outputs
Outputs of similar age
#21,729
of 336,374 outputs
Outputs of similar age from Nature Communications
#428
of 1,231 outputs
Altmetric has tracked 24,940,046 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 54,625 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 55.8. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 336,374 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 1,231 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.