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microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb

Overview of attention for article published in Brazilian Journal of Medical and Biological Research, January 2018
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Title
microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb
Published in
Brazilian Journal of Medical and Biological Research, January 2018
DOI 10.1590/1414-431x20186452
Pubmed ID
Authors

Shaofeng Yang, Nan Sheng, Lili Pan, Jing Cao, Jiao Liu, Ran Ma

Abstract

Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A significant up-regulation of miR-3129 was observed in GC tissues compared to adjacent tissues. Overexpression of miR-3129 significantly improved cell viability after 4 days of post-transfection. Flow cytometry assay results showed that the miR-3129 overexpression arrested more SGC7901 cells at S phase. Moreover, overexpression of miR-3129 down-regulated the expression of CDK2 inhibitors while it up-regulated the expression levels of cyclin E, CDK2, and pRb. Interestingly, we found that pRb inhibition reversed the effect of miR-3129 inhibitor on cell proliferation in SGC7901 cells, increased cell viability, reduced cells at G0/1 phase, and modulated the expression of proliferation-related factors. Our results revealed that miR-3129 functioned as an oncogene through positive regulation of pRb and may prove to be a promising option for molecular therapy of GC.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 25%
Lecturer 1 13%
Student > Doctoral Student 1 13%
Student > Bachelor 1 13%
Professor 1 13%
Other 0 0%
Unknown 2 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Nursing and Health Professions 1 13%
Social Sciences 1 13%
Medicine and Dentistry 1 13%
Unknown 3 38%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 May 2018.
All research outputs
#19,069,905
of 21,431,229 outputs
Outputs from Brazilian Journal of Medical and Biological Research
#528
of 580 outputs
Outputs of similar age
#261,527
of 299,809 outputs
Outputs of similar age from Brazilian Journal of Medical and Biological Research
#4
of 4 outputs
Altmetric has tracked 21,431,229 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 580 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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