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stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells

Overview of attention for article published in Biological Research, July 2015
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Title
stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells
Published in
Biological Research, July 2015
DOI 10.1186/s40659-015-0024-9
Pubmed ID
Authors

Liliana Berrocal, Juan A Fuentes, A Nicole Trombert, Matías R Jofré, Nicolás A Villagra, Luis M Valenzuela, Guido C Mora

Abstract

Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by the lower level of invasion to macrophage-like cells observed when the stg fimbrial cluster was overexpressed. Nevertheless, since other CU fimbrial structures increase the entry of S. Typhi into macrophages, and considering that transcriptomic analyses revealed that stg operon is indeed expressed in macrophages, we reassessed the role of the stg operon in the interaction between S. Typhi strain STH2370 and human cells, including macrophage-like cells and mononuclear cells directly taken from human peripheral blood. We compared S. Typhi STH2370 WT, a Chilean clinical strain, and the S. Typhi STH2370 Δstg mutant with respect to association and invasion using epithelial and macrophage-like cells. We observed that deletion of stg operon reduced the association and invasion of S. Typhi, in both cellular types. The presence of the cloned stg operon restored the WT phenotype in all the cases. Moreover, we compared Salmonella enterica sv. Typhimurium 14028s (S. Typhimurium, a serovar lacking stg operon) and S. Typhimurium heterologously expressing S. Typhi stg. We found that the latter presents an increased cell disruption of polarized epithelial cells and an increased association in both epithelial and macrophage-like cells. S. Typhi stg operon encodes a functional adhesin that participates in the interaction bacteria-eukaryotic cells, including epithelial cells and macrophages-like cells. The phenotypes associated to stg operon include increased association and consequent invasion in bacteria-eukaryotic cells, and cell disruption.

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The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 33%
Student > Master 2 17%
Student > Ph. D. Student 2 17%
Student > Doctoral Student 1 8%
Professor 1 8%
Other 2 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 42%
Immunology and Microbiology 3 25%
Biochemistry, Genetics and Molecular Biology 2 17%
Veterinary Science and Veterinary Medicine 1 8%
Unknown 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 July 2015.
All research outputs
#3,749,341
of 5,325,534 outputs
Outputs from Biological Research
#114
of 188 outputs
Outputs of similar age
#129,432
of 185,954 outputs
Outputs of similar age from Biological Research
#10
of 20 outputs
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So far Altmetric has tracked 188 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
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