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Diagnosis of bacteremia in pediatric oncologic patients by in-house real-time PCR

Overview of attention for article published in BMC Infectious Diseases, July 2015
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Title
Diagnosis of bacteremia in pediatric oncologic patients by in-house real-time PCR
Published in
BMC Infectious Diseases, July 2015
DOI 10.1186/s12879-015-1033-6
Pubmed ID
Authors

Milene Gonçalves Quiles, Liana Carballo Menezes, Karen de Castro Bauab, Elke Kreuscher Gumpl, Talita Trevizani Rocchetti, Flavia Silva Palomo, Fabianne Carlesse, Antonio Carlos Campos Pignatari

Abstract

Infections are the major cause of morbidity and mortality in children with cancer. Gaining a favorable prognosis for these patients depends on selecting the appropriate therapy, which in turn depends on rapid and accurate microbiological diagnosis. This study employed real-time PCR (qPCR) to identify the main pathogens causing bloodstream infection (BSI) in patients treated at the Pediatric Oncology Institute IOP-GRAACC-UNIFESP-Brazil. Antimicrobial resistance genes were also investigated using this methodology. A total of 248 samples from BACTEC® blood culture bottles and 99 whole-blood samples collected in tubes containing EDTA K2 Gel were isolated from 137 patients. All samples were screened by specific Gram probes for multiplex qPCR. Seventeen sequences were evaluated using gender-specific TaqMan probes and the resistance genes bla SHV, bla TEM, bla CTX, bla KPC, bla IMP, bla SPM, bla VIM, vanA, vanB and mecA were detected using the SYBR Green method. Positive qPCR results were obtained in 112 of the blood culture bottles (112/124), and 90 % agreement was observed between phenotypic and molecular microbial detection methods. For bacterial and fungal identification, the performance test showed: sensitivity 87 %; specificity 91 %; NPV 90 %; PPV 89 % and accuracy of 89 % when compared with the phenotypic method. The mecA gene was detected in 37 samples, extended-spectrum β-lactamases were detected in six samples and metallo-β-lactamase coding genes in four samples, with 60 % concordance between the two methods. The qPCR on whole blood detected eight samples possessing the mecA gene and one sample harboring the vanB gene. The bla KPC, bla VIM, bla IMP and bla SHV genes were not detected in this study. Real-time PCR is a useful tool in the early identification of pathogens and antimicrobial resistance genes from bloodstream infections of pediatric oncologic patients.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 49 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 16%
Student > Master 8 16%
Researcher 7 14%
Student > Doctoral Student 6 12%
Student > Bachelor 6 12%
Other 8 16%
Unknown 7 14%
Readers by discipline Count As %
Medicine and Dentistry 19 38%
Agricultural and Biological Sciences 6 12%
Biochemistry, Genetics and Molecular Biology 5 10%
Nursing and Health Professions 3 6%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Other 4 8%
Unknown 11 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2015.
All research outputs
#3,795,387
of 5,393,711 outputs
Outputs from BMC Infectious Diseases
#2,108
of 2,839 outputs
Outputs of similar age
#131,605
of 189,662 outputs
Outputs of similar age from BMC Infectious Diseases
#121
of 149 outputs
Altmetric has tracked 5,393,711 research outputs across all sources so far. This one is in the 16th percentile – i.e., 16% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 149 others from the same source and published within six weeks on either side of this one. This one is in the 2nd percentile – i.e., 2% of its contemporaries scored the same or lower than it.