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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Neurotensin-polyplex-mediated brain-derived neurotrophic factor gene delivery into nigral dopamine neurons prevents nigrostriatal degeneration in a rat model of early Parkinson’s disease
|
|---|---|
| Published in |
Journal of Biomedical Science, July 2015
|
| DOI | 10.1186/s12929-015-0166-7 |
| Pubmed ID | |
| Authors |
Nancy G. Hernandez-Chan, Michael J. Bannon, Carlos E. Orozco-Barrios, Lourdes Escobedo, Sergio Zamudio, Fidel De la Cruz, Jose L. Gongora-Alfaro, Juan Armendáriz-Borunda, David Reyes-Corona, Armando J. Espadas-Alvarez, Yazmin M. Flores-Martínez, Jose Ayala-Davila, Maria E. Hernandez-Gutierrez, Lenin Pavón, Refugio García-Villegas, Rasajna Nadella, Daniel Martinez-Fong |
| Abstract |
The neurotrophin Brain-Derived Neurotrophic Factor (BDNF) influences nigral dopaminergic neurons via autocrine and paracrine mechanisms. The reduction of BDNF expression in Parkinson's disease substantia nigra (SN) might contribute to the death of dopaminergic neurons because inhibiting BDNF expression in the SN causes parkinsonism in the rat. This study aimed to demonstrate that increasing BDNF expression in dopaminergic neurons of rats with one week of 6-hydroxydopamine lesion recovers from parkinsonism. The plasmids phDAT-BDNF-flag and phDAT-EGFP, coding for enhanced green fluorescent protein, were transfected using neurotensin (NTS)-polyplex, which enables delivery of genes into the dopaminergic neurons via neurotensin-receptor type 1 (NTSR1) internalization. Two weeks after transfections, RT-PCR and immunofluorescence techniques showed that the residual dopaminergic neurons retain NTSR1 expression and susceptibility to be transfected by the NTS-polyplex. phDAT-BDNF-flag transfection did not increase dopaminergic neurons, but caused 7-fold increase in dopamine fibers within the SN and 5-fold increase in innervation and dopamine levels in the striatum. These neurotrophic effects were accompanied by a significant improvement in motor behavior. NTS-polyplex-mediated BDNF overexpression in dopaminergic neurons has proven to be effective to remit hemiparkinsonism in the rat. This BDNF gene therapy might be helpful in the early stage of Parkinson's disease. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 70 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 15 | 21% |
| Student > Bachelor | 8 | 11% |
| Researcher | 6 | 9% |
| Other | 5 | 7% |
| Professor | 5 | 7% |
| Other | 12 | 17% |
| Unknown | 19 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 16% |
| Medicine and Dentistry | 9 | 13% |
| Agricultural and Biological Sciences | 9 | 13% |
| Neuroscience | 8 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
| Other | 10 | 14% |
| Unknown | 20 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2015.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Journal of Biomedical Science
#753
of 1,101 outputs
Outputs of similar age
#164,206
of 275,278 outputs
Outputs of similar age from Journal of Biomedical Science
#13
of 22 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,101 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.0. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,278 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.