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Overview of attention for article published in BMC Biotechnology, January 2002
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (77th percentile)

Mentioned by

blogs
1 blog

Citations

dimensions_citation
204 Dimensions

Readers on

mendeley
129 Mendeley
connotea
1 Connotea
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Title
Published in
BMC Biotechnology, January 2002
DOI 10.1186/1472-6750-2-11
Pubmed ID
Authors

Anna-Katerina Hadjantonakis, Suzanne Macmaster, Andras Nagy

Abstract

Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes 1. It offers several advantages over conventional gene-based reporters, such as lacZ and alkaline phosphatase, in that its visualization does not require a chromogenic substrate and can be realized in vivo. We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice 2. In this study we have used embryonic stem (ES) cell-mediated transgenesis to test the enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP), two mutant and spectrally distinct color variants of wild type (wt) GFP. We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP. To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters. However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively. Balanced (diploid <-> diploid) and polarized (tetraploid <-> diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal. GFP variant reporters are unique in allowing non-invasive multi-spectral visualization in live samples. The ECFP and EYFP-expressing transgenic ES cells and mice that we have generated provide sources of cells and tissues for combinatorial, double-tagged recombination experiments, chimeras or transplantations.

Mendeley readers

The data shown below were compiled from readership statistics for 129 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 4 3%
United States 3 2%
Canada 2 2%
Vietnam 1 <1%
Ireland 1 <1%
Iran, Islamic Republic of 1 <1%
Spain 1 <1%
Australia 1 <1%
Unknown 115 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 34 26%
Student > Ph. D. Student 26 20%
Student > Master 15 12%
Professor > Associate Professor 12 9%
Student > Bachelor 9 7%
Other 28 22%
Unknown 5 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 71 55%
Biochemistry, Genetics and Molecular Biology 21 16%
Medicine and Dentistry 9 7%
Neuroscience 7 5%
Immunology and Microbiology 4 3%
Other 10 8%
Unknown 7 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 July 2015.
All research outputs
#2,735,844
of 12,373,386 outputs
Outputs from BMC Biotechnology
#215
of 695 outputs
Outputs of similar age
#52,857
of 237,145 outputs
Outputs of similar age from BMC Biotechnology
#3
of 4 outputs
Altmetric has tracked 12,373,386 research outputs across all sources so far. Compared to these this one has done well and is in the 77th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 695 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 237,145 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one.