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Mesothelioma patient derived tumor xenografts with defined BAP1 mutations that mimic the molecular characteristics of human malignant mesothelioma

Overview of attention for article published in BMC Cancer, May 2015
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Title
Mesothelioma patient derived tumor xenografts with defined BAP1 mutations that mimic the molecular characteristics of human malignant mesothelioma
Published in
BMC Cancer, May 2015
DOI 10.1186/s12885-015-1362-2
Pubmed ID
Authors

Neetu Kalra, Jingli Zhang, Anish Thomas, Liqiang Xi, Mitchell Cheung, Jacqueline Talarchek, Sandra Burkett, Maria G Tsokos, Yuanbin Chen, Mark Raffeld, Markku Miettinen, Ira Pastan, Joseph R Testa, Raffit Hassan

Abstract

The development and evaluation of new therapeutic approaches for malignant mesothelioma has been sparse due, in part, to lack of suitable tumor models. We established primary mesothelioma cultures from pleural and ascites fluids of five patients with advanced mesothelioma. Electron microscopy and immunohistochemistry (IHC) confirmed their mesothelial origin. Patient derived xenografts were generated by injecting the cells in nude or SCID mice, and malignant potential of the cells was analyzed by soft agar colony assay. Molecular profiles of the primary patient tumors, early passage cell cultures, and patient derived xenografts were assessed using mutational analysis, fluorescence in situ hybridization (FISH) analysis and IHC. Primary cultures from all five tumors exhibited morphologic and IHC features consistent to those of mesothelioma cells. Mutations of BAP1 and CDKN2A were each detected in four tumors. BAP1 mutation was associated with the lack of expression of BAP1 protein. Three cell cultures, all of which were derived from BAP1 mutant primary tumors, exhibited anchorage independent growth and also formed tumors in mice, suggesting that BAP1 loss may enhance tumor growth in vivo. Both early passage cell cultures and mouse xenograft tumors harbored BAP1 mutations and CDKN2A deletions identical to those found in the corresponding primary patient tumors. The mesothelioma patient derived tumor xenografts with mutational alterations that mimic those observed in patient tumors which we established can be used for preclinical development of novel drug regimens and for studying the functional aspects of BAP1 biology in mesothelioma.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 4%
Unknown 22 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 30%
Student > Ph. D. Student 3 13%
Student > Master 3 13%
Student > Doctoral Student 2 9%
Professor 2 9%
Other 4 17%
Unknown 2 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 30%
Medicine and Dentistry 6 26%
Agricultural and Biological Sciences 4 17%
Nursing and Health Professions 2 9%
Chemistry 1 4%
Other 0 0%
Unknown 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 July 2015.
All research outputs
#4,440,865
of 5,333,889 outputs
Outputs from BMC Cancer
#2,231
of 2,772 outputs
Outputs of similar age
#150,793
of 187,692 outputs
Outputs of similar age from BMC Cancer
#147
of 153 outputs
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We're also able to compare this research output to 153 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.