RT @ChampionNd: @ATinyGreenCell Nothing. Fast cloning /slice cloning does this. 15bp is generally the minimum. Above 3 pieces though E. col…
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@ATinyGreenCell Nothing. Fast cloning /slice cloning does this. 15bp is generally the minimum. Above 3 pieces though E. coli tends to go all restriction mod on it and degrade the DNA. Best efficiency is in rec - strains like XL1 Blue. https://t.co/0
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RT @bryochemist: @ATinyGreenCell We refer to those two papers when designing our primers and the transformation step. (Better than) QuikCh…
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@ATinyGreenCell We refer to those two papers when designing our primers and the transformation step. (Better than) QuikChange: https://t.co/GCmgcNnLu2 FastCloning: https://t.co/CbjzJCgSAi