| Chapter title |
Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing Without A Priori Viral Diversity of Plants.
|
|---|---|
| Chapter number | 18 |
| Book title |
Plant Pathology
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2620-6_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2619-0, 978-1-4939-2620-6
|
| Authors |
Filloux, Denis, Dallot, Sylvie, Delaunay, Agnès, Galzi, Serge, Jacquot, Emmanuel, Roumagnac, Philippe, Denis Filloux, Sylvie Dallot, Agnès Delaunay, Serge Galzi, Emmanuel Jacquot, Philippe Roumagnac |
| Abstract |
This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 6% |
| Unknown | 32 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 21% |
| Researcher | 6 | 18% |
| Student > Doctoral Student | 4 | 12% |
| Student > Ph. D. Student | 4 | 12% |
| Student > Bachelor | 3 | 9% |
| Other | 5 | 15% |
| Unknown | 5 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 47% |
| Biochemistry, Genetics and Molecular Biology | 7 | 21% |
| Unspecified | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 7 | 21% |