| Chapter title |
Plant Pathology
|
|---|---|
| Chapter number | 21 |
| Book title |
Plant Pathology
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2620-6_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2619-0, 978-1-4939-2620-6
|
| Authors |
van der Vlugt, René A A, van Raaij, Henry, de Weerdt, Marjanne, Bergervoet, Jan H W, René A. A. van der Vlugt, Henry van Raaij, Marjanne de Weerdt, Jan H. W. Bergervoet |
| Abstract |
Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres ("beads"), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or bacteria or PCR products obtained from their genetic material.The Luminex MagPlex bead system allows true multiplex detection of up to 500 targets in a single sample on a routine basis. The liquid suspension nature of the method significantly improves (1) assay speed, (2) detection limits and (3) dynamic range. It can also considerably reduce labor and consumables costs. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 11% |
| Unknown | 8 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 44% |
| Student > Ph. D. Student | 3 | 33% |
| Student > Bachelor | 1 | 11% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 33% |
| Medicine and Dentistry | 2 | 22% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Immunology and Microbiology | 1 | 11% |
| Neuroscience | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |