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Plant Pathology

Overview of attention for book
Cover of 'Plant Pathology'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Plant Pathology
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    Chapter 2 Detection and Identification of Phoma Pathogens of Potato
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    Chapter 3 Plant Pathology
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    Chapter 4 A real-time multiplex PCR assay used in the identification of closely related fungal pathogens at the species level.
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    Chapter 5 Diagnostics of Tree Diseases Caused by Phytophthora austrocedri Species
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    Chapter 6 Real-Time LAMP for Chalara fraxinea Diagnosis
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    Chapter 7 Plant Pathology
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    Chapter 8 Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field
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    Chapter 9 Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes
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    Chapter 10 Q-Bank Phytoplasma: A DNA Barcoding Tool for Phytoplasma Identification
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    Chapter 11 Plant Pathology
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    Chapter 12 Plant Pathology
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    Chapter 13 Plant Pathology
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    Chapter 14 Plant Pathology
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    Chapter 15 SNaPshot and CE-SSCP: Two Simple and Cost-Effective Methods to Reveal Genetic Variability Within a Virus Species
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    Chapter 16 Detection and Characterization of Viral Species/Subspecies Using Isothermal Recombinase Polymerase Amplification (RPA) Assays.
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    Chapter 17 Virus Testing by PCR and RT-PCR Amplification in Berry Fruit
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    Chapter 18 Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing Without A Priori Viral Diversity of Plants.
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    Chapter 19 Plant Pathology
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    Chapter 20 Microarray Platform for the Detection of a Range of Plant Viruses and Viroids
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    Chapter 21 Plant Pathology
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    Chapter 22 Next-Generation Sequencing of Elite Berry Germplasm and Data Analysis Using a Bioinformatics Pipeline for Virus Detection and Discovery
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    Chapter 23 Metagenomic next-generation sequencing of viruses infecting grapevines.
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    Chapter 24 Droplet Digital PCR for Absolute Quantification of Pathogens
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    Chapter 25 Erratum to: Diagnostics of Tree Diseases Caused by Phytophthora austrocedri Species
Attention for Chapter 14: Plant Pathology
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Chapter title
Plant Pathology
Chapter number 14
Book title
Plant Pathology
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2620-6_14
Pubmed ID
Book ISBNs
978-1-4939-2619-0, 978-1-4939-2620-6
Authors

Chikh-Ali, Mohamad, Karasev, Alexander V, Mohamad Chikh-Ali, Alexander V. Karasev

Abstract

Immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) is a sensitive, reproducible, and robust method for the detection and identification of RNA viruses. The IC step provides a simple method to isolate virus particles from plant tissue, particularly when inhibitory substances are present, and thus enables subsequent use of RT-PCR amplification for large-scale virus testing and typing. The multiplex format of the PCR is often used for the detection and identification of multiple virus/strain simultaneously to save time, labor, and cost. Potato virus Y (PVY) is one of the most economically important viruses infecting potato worldwide. PVY exists as a complex of at least nine strains and many more unclassified recombinants that vary in their genome structures, phenotypes, and their economic importance. In the current chapter, a detailed protocol of an IC-based, multiplex RT-PCR assay for the detection and identification of various PVY strains is described.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Other 4 31%
Student > Ph. D. Student 2 15%
Researcher 2 15%
Student > Master 1 8%
Student > Bachelor 1 8%
Other 0 0%
Unknown 3 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 38%
Biochemistry, Genetics and Molecular Biology 1 8%
Chemistry 1 8%
Unknown 6 46%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 March 2016.
All research outputs
#20,315,221
of 22,856,968 outputs
Outputs from Methods in molecular biology
#9,917
of 13,128 outputs
Outputs of similar age
#295,975
of 353,259 outputs
Outputs of similar age from Methods in molecular biology
#636
of 997 outputs
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