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Selection of reference genes for quantitative real-time PCR normalization in creeping bentgrass involved in four abiotic stresses

Overview of attention for article published in Plant Cell Reports, July 2015
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Title
Selection of reference genes for quantitative real-time PCR normalization in creeping bentgrass involved in four abiotic stresses
Published in
Plant Cell Reports, July 2015
DOI 10.1007/s00299-015-1830-9
Pubmed ID
Authors

Yu Chen, Baoyun Hu, Zhiqun Tan, Jun Liu, Zhimin Yang, Zhihua Li, Bingru Huang

Abstract

This study identified stable reference genes for normalization of gene expression data in qRT-PCR analysis of leaf and root tissues in creeping bentgrass under four abiotic stresses. Examination of gene expression using quantitative real-time PCR (qRT-PCR) in plant responses to abiotic stresses can provide valuable information for stress-tolerance improvement. Selecting stable reference genes for qRT-PCR analysis is critically important. The objective of this study was to determine the stability of expression for eight candidate reference genes (ACT, EF1a, TUB, UPL7, GAPDH, PP2A, PEPKR1, and CACS) in two tissues (roots and leaves) of a perennial grass species under four abiotic stresses (salt, drought, cold, and heat) using four programs (GeNorm, NormFinder, BestKeeper, and RefFinder). The results showed that (1) the combinations of CACS and UPL7 or PP2A and ACT were stably expressed in salt-treated roots or leaves; (2) the combinations of GAPDH and CACS or PP2A and PEPKR1 were stable in roots and leaves under drought stress; (3) CACS and PP2A exhibited stable expression in cold-treated roots and the combination of EF1a and UPL7 was also stable in cold-treated leaves; and (4) CACS and PP2A were the two most stable reference genes in heat-stressed roots and UPL7 combined with GAPDH and PP2A was stably expressed in heat-stressed leaves. The qRT-PCR analysis of a target gene, AsSAP expression patterns in response to salinity and drought stress, confirmed the reliability of those selected and stable reference genes. Identification of stable reference genes in creeping bentgrass will improve assay accuracy for selecting stress-tolerance genes and identifying molecular mechanisms conferring stress tolerance in this species.

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Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 25%
Student > Master 2 17%
Student > Postgraduate 1 8%
Lecturer 1 8%
Student > Bachelor 1 8%
Other 4 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 58%
Unspecified 3 25%
Biochemistry, Genetics and Molecular Biology 1 8%
Engineering 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2015.
All research outputs
#9,769,916
of 12,226,671 outputs
Outputs from Plant Cell Reports
#1,181
of 1,504 outputs
Outputs of similar age
#165,224
of 238,028 outputs
Outputs of similar age from Plant Cell Reports
#18
of 49 outputs
Altmetric has tracked 12,226,671 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,504 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 238,028 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.