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Serotonin improves glucose metabolism by Serotonylation of the small GTPase Rab4 in L6 skeletal muscle cells

Overview of attention for article published in Diabetology & Metabolic Syndrome, January 2017
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Title
Serotonin improves glucose metabolism by Serotonylation of the small GTPase Rab4 in L6 skeletal muscle cells
Published in
Diabetology & Metabolic Syndrome, January 2017
DOI 10.1186/s13098-016-0201-1
Pubmed ID
Authors

Ramona Al-Zoairy, Michael T. Pedrini, Mohammad Imran Khan, Julia Engl, Alexander Tschoner, Christoph Ebenbichler, Gerhard Gstraunthaler, Karin Salzmann, Rania Bakry, Andreas Niederwanger

Abstract

Serotonin (5-HT) improves insulin sensitivity and glucose metabolism, however, the underlying molecular mechanism has remained elusive. Previous studies suggest that 5-HT can activate intracellular small GTPases directly by covalent binding, a process termed serotonylation. Activated small GTPases have been associated with increased GLUT4 translocation to the cell membrane. Therefore, we investigated whether serotonylation of small GTPases may be involved in improving Insulin sensitivity and glucose metabolism. Using fully differentiated L6 rat skeletal muscle cells, we studied the effect of 5-HT in the absence or presence of insulin on glycogen synthesis, glucose uptake and GLUT4 translocation. To prove our L6 model we additionally performed preliminary experiments in C2C12 murine skeletal muscle cells. Incubation with 5-HT led to an increase in deoxyglucose uptake in a concentration-dependent fashion. Accordingly, GLUT4 translocation to the cell membrane and glycogen content were increased. These effects of 5-HT on Glucose metabolism could be augmented by co-incubation with insulin and blunted by co incubation of 5-HT with monodansylcadaverine, an inhibitor of protein serotonylation. In accordance with this observation, incubation with 5-HT resulted in serotonylation of a protein with a molecular weight of approximately 25 kDa. We identified this protein as the small GTPase Rab4, the activity of which has been shown to be stimulated by both insulin signalling and serotonylation. Our data suggest that 5-HT elicits its beneficial effects on Glucose metabolism through serotonylation of Rab4, which likely represents the converging point between the insulin and the 5-HT signalling cascades.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 24%
Student > Ph. D. Student 6 18%
Researcher 3 9%
Student > Bachelor 3 9%
Professor 2 6%
Other 2 6%
Unknown 9 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 27%
Pharmacology, Toxicology and Pharmaceutical Science 4 12%
Neuroscience 3 9%
Agricultural and Biological Sciences 2 6%
Medicine and Dentistry 1 3%
Other 1 3%
Unknown 13 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2018.
All research outputs
#15,536,861
of 23,090,520 outputs
Outputs from Diabetology & Metabolic Syndrome
#374
of 678 outputs
Outputs of similar age
#257,855
of 422,116 outputs
Outputs of similar age from Diabetology & Metabolic Syndrome
#6
of 8 outputs
Altmetric has tracked 23,090,520 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 678 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.2. This one is in the 34th percentile – i.e., 34% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 422,116 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.