Title |
One nanoprobe, two pathogens: gold nanoprobes multiplexing for point-of-care
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Published in |
Journal of Nanobiotechnology, August 2015
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DOI | 10.1186/s12951-015-0109-1 |
Pubmed ID | |
Authors |
Bruno Veigas, Pedro Pedrosa, Fábio F Carlos, Liliana Mancio-Silva, Ana Rita Grosso, Elvira Fortunato, Maria M Mota, Pedro V Baptista |
Abstract |
Gold nanoparticles have been widely employed for biosensing purposes with remarkable efficacy for DNA detection. Amongst the proposed systems, colorimetric strategies based on the remarkable optical properties have provided for simple yet effective sequence discrimination with potential for molecular diagnostics at point of need. These systems may also been used for parallel detection of several targets to provide additional information on diagnostics of pathogens. For the first time, we demonstrate that a single Au-nanoprobe may provide for detection of two distinct targets (pathogens) allowing colorimetric multi-target detection. We demonstrate this concept by using one single gold-nanoprobe capable to detect members of the Mycobacterium tuberculosis complex and Plasmodium sp., the etiologic agents of tuberculosis and malaria, respectively. Following characterisation, the developed gold-nanoprobe allowed detection of either target in individual samples or in samples containing both DNA species with the same efficacy. Using one single probe via the non-cross-linking colorimetric methodology it is possible to identify multiple targets in one sample in one reaction. This proof-of-concept approach may easily be integrated into sensing platforms allowing for fast and simple multiplexing of Au-nanoprobe based detection at point-of-need. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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India | 1 | 2% |
Unknown | 52 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 9 | 17% |
Student > Ph. D. Student | 6 | 11% |
Student > Bachelor | 6 | 11% |
Student > Master | 6 | 11% |
Student > Doctoral Student | 5 | 9% |
Other | 10 | 19% |
Unknown | 11 | 21% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 12 | 23% |
Biochemistry, Genetics and Molecular Biology | 9 | 17% |
Engineering | 6 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
Medicine and Dentistry | 2 | 4% |
Other | 7 | 13% |
Unknown | 15 | 28% |