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Detection, identification and genotyping of Borrellia spp. in rodents in Slovenia by PCR and culture

Overview of attention for article published in BMC Veterinary Research, August 2015
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  • Above-average Attention Score compared to outputs of the same age and source (58th percentile)

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Title
Detection, identification and genotyping of Borrellia spp. in rodents in Slovenia by PCR and culture
Published in
BMC Veterinary Research, August 2015
DOI 10.1186/s12917-015-0501-y
Pubmed ID
Authors

Tjaša Cerar, Miša Korva, Tatjana Avšič-Županc, Eva Ružić-Sabljić

Abstract

Borrelia burgdorferi sensu lato, the agent of Lyme borreliosis, is mainly maintained in natural foci through the transmission cycles of competent tick vectors (Ixodes sp.) and a vertebrate reservoir. Specific rodents have been identified as the principal reservoir of Borrelia burgdorferi sensu lato in Europe. Borrelia miyamotoi is the only relapsing fever spirochete transmitted by the same tick. The aim of the present study was to perform an epidemiological survey to determine the presence of B. burgdorferi sensu lato in rodents occurring in Slovenia and to explore the presence of Borrelia miyamotoi. The study was performed in two parts, retrospective and prospective; a total of 297 rodents was analyzed. Detection and identification of borrelia was performed by molecular methods and additionally in the prospective study by isolation and genotyping (MluI-LRFP and MLST). During the prospective part of the study, borrelia was isolated from 2/46 (4.3 %) lung specimens and from 10/46 (21.7 %) heart specimens of rodents. All isolated strains were identified as B. afzelii subtype Mla1, and MLST analysis revealed 5 distinct sequence types. Borrelia DNA was successfully detected by one or other of the PCR methods in 18/46 (39.1 %) and 75/251 (29.9 %) samples in the prospective and retrospective studies, respectively. LightMix® was found to be more sensitive than the ''in-house" nested PCR (91/297 (30.6 %) vs 48/297 (16.1 %)). Borrelia miyamotoi DNA was detected in 1/251 (0.4 %) and in 1/46 (2.2 %) heart specimens, in the retrospective and prospective parts of the study, respectively. We determined the prevalence of B. afzelii in rodents and report for the first time the presence of B. miyamotoi in Slovenia.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
United States 1 3%
Colombia 1 3%
Unknown 35 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 18%
Other 5 13%
Student > Doctoral Student 4 11%
Student > Master 4 11%
Researcher 4 11%
Other 7 18%
Unknown 7 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 32%
Veterinary Science and Veterinary Medicine 6 16%
Biochemistry, Genetics and Molecular Biology 5 13%
Philosophy 2 5%
Medicine and Dentistry 2 5%
Other 3 8%
Unknown 8 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2015.
All research outputs
#14,234,315
of 22,821,814 outputs
Outputs from BMC Veterinary Research
#1,110
of 3,050 outputs
Outputs of similar age
#136,384
of 264,425 outputs
Outputs of similar age from BMC Veterinary Research
#27
of 70 outputs
Altmetric has tracked 22,821,814 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,050 research outputs from this source. They receive a mean Attention Score of 3.8. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,425 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 70 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.