Title |
Loop-mediated isothermal amplification for detection of porcine circovirus type 2
|
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Published in |
Virology Journal, November 2011
|
DOI | 10.1186/1743-422x-8-497 |
Pubmed ID | |
Authors |
Shun Zhou, Si Han, Jianli Shi, Jiaqiang Wu, Xiaoyuan Yuan, Xiaoyan Cong, Shaojian Xu, Xiaoyan Wu, Jun Li, Jinbao Wang |
Abstract |
Porcine circovirus type 2 (PCV2) is the primary causative agent of the emerging swine disease known as postweaning multisystemic wasting syndrome (PMWS). Nowadays, polymerase chain reaction (PCR) is still the most widespread technique in pathogen detection. Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method developed in 2000, will possibly replace PCR in the field of detection. To establish a LAMP method for rapid detection of PCV2, two pairs of primers were designed specially from the open reading frame 2 (ORF2) sequences of PCV2. A LAMP method for rapid detection of PCV2 was established. To compare with PCR, sensitivity and specificity of LAMP were evaluated using the optimized reaction system. The LAMP products could be determined by agarose gel electrophoresis or adding SYBR Green I dye. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Kenya | 1 | 3% |
Ukraine | 1 | 3% |
Denmark | 1 | 3% |
Greece | 1 | 3% |
Madagascar | 1 | 3% |
Unknown | 27 | 84% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 7 | 22% |
Researcher | 4 | 13% |
Student > Ph. D. Student | 4 | 13% |
Student > Bachelor | 4 | 13% |
Professor > Associate Professor | 3 | 9% |
Other | 7 | 22% |
Unknown | 3 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 31% |
Biochemistry, Genetics and Molecular Biology | 7 | 22% |
Medicine and Dentistry | 5 | 16% |
Veterinary Science and Veterinary Medicine | 4 | 13% |
Unspecified | 1 | 3% |
Other | 0 | 0% |
Unknown | 5 | 16% |