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Loop-mediated isothermal amplification for detection of porcine circovirus type 2

Overview of attention for article published in Virology Journal, November 2011
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Title
Loop-mediated isothermal amplification for detection of porcine circovirus type 2
Published in
Virology Journal, November 2011
DOI 10.1186/1743-422x-8-497
Pubmed ID
Authors

Shun Zhou, Si Han, Jianli Shi, Jiaqiang Wu, Xiaoyuan Yuan, Xiaoyan Cong, Shaojian Xu, Xiaoyan Wu, Jun Li, Jinbao Wang

Abstract

Porcine circovirus type 2 (PCV2) is the primary causative agent of the emerging swine disease known as postweaning multisystemic wasting syndrome (PMWS). Nowadays, polymerase chain reaction (PCR) is still the most widespread technique in pathogen detection. Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method developed in 2000, will possibly replace PCR in the field of detection. To establish a LAMP method for rapid detection of PCV2, two pairs of primers were designed specially from the open reading frame 2 (ORF2) sequences of PCV2. A LAMP method for rapid detection of PCV2 was established. To compare with PCR, sensitivity and specificity of LAMP were evaluated using the optimized reaction system. The LAMP products could be determined by agarose gel electrophoresis or adding SYBR Green I dye.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Kenya 1 3%
Ukraine 1 3%
Denmark 1 3%
Greece 1 3%
Madagascar 1 3%
Unknown 27 84%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 22%
Researcher 4 13%
Student > Ph. D. Student 4 13%
Student > Bachelor 4 13%
Professor > Associate Professor 3 9%
Other 7 22%
Unknown 3 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 31%
Biochemistry, Genetics and Molecular Biology 7 22%
Medicine and Dentistry 5 16%
Veterinary Science and Veterinary Medicine 4 13%
Unspecified 1 3%
Other 0 0%
Unknown 5 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 November 2011.
All research outputs
#15,238,442
of 22,656,971 outputs
Outputs from Virology Journal
#1,932
of 3,022 outputs
Outputs of similar age
#96,461
of 141,797 outputs
Outputs of similar age from Virology Journal
#51
of 91 outputs
Altmetric has tracked 22,656,971 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,022 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.5. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 141,797 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 91 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.