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Nitric oxide stimulates early egress of Toxoplasma gondii tachyzoites from human foreskin fibroblast cells

Overview of attention for article published in Parasites & Vectors, August 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (77th percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

Mentioned by

blogs
1 blog

Citations

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8 Dimensions

Readers on

mendeley
13 Mendeley
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Title
Nitric oxide stimulates early egress of Toxoplasma gondii tachyzoites from human foreskin fibroblast cells
Published in
Parasites & Vectors, August 2015
DOI 10.1186/s13071-015-1037-5
Pubmed ID
Authors

Xinlei Yan, Yongsheng Ji, Xianyong Liu, Xun Suo

Abstract

Egress is a vital step in the life cycle of Toxoplasma gondii which attracts attentions of many groups. Previous studies have shown that exogenous nitric oxide (NO) stimulates the early egress of T. gondii from infected peritoneal macrophages, a kind of immune cells. However, because Toxoplasma forms cysts in brain and muscle tissues, the development of autonomous immunity in non-immune cells is vital for limiting parasite burden and cyst formation. Therefore, we attempted to investigate whether exogenous NO could induce the early egress of T. gondii from infected non-immune cells. T. gondii tachyzoites were cultured in human foreskin fibroblast (HFF) cells and were then treated with NO released by sodium nitroferricyanide (III) dihydrate (SNP). The egressed parasites were analysed by flow cytometry. The results showed that NO induced the early egress of parasites from HFF cells before completing their intracellular life cycles. We also found that the occurrence of egress was dependent on intracellular calcium (Ca(2+)) levels and the mobility of the parasite. Compared with freshly isolated tachyzoites, the developmental ability and virulence of egressed tachyzoites presented no difference. Taken together, our findings demonstrate a novel assay for the analysis of egress signalling mechanisms and an avenue of parasite clearance by hosts of T. gondii.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 38%
Student > Master 2 15%
Other 2 15%
Student > Postgraduate 1 8%
Student > Bachelor 1 8%
Other 2 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 46%
Unspecified 2 15%
Biochemistry, Genetics and Molecular Biology 2 15%
Immunology and Microbiology 1 8%
Neuroscience 1 8%
Other 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 August 2015.
All research outputs
#1,797,734
of 8,837,009 outputs
Outputs from Parasites & Vectors
#399
of 2,525 outputs
Outputs of similar age
#52,863
of 231,084 outputs
Outputs of similar age from Parasites & Vectors
#12
of 117 outputs
Altmetric has tracked 8,837,009 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,525 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,084 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 117 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.