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Transcriptome profile analysis reflects rat liver and kidney damage following chronic ultra-low dose Roundup exposure

Overview of attention for article published in Environmental Health, August 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#11 of 1,119)
  • High Attention Score compared to outputs of the same age (99th percentile)

Mentioned by

news
15 news outlets
blogs
6 blogs
twitter
309 tweeters
peer_reviews
1 peer review site
facebook
68 Facebook pages
wikipedia
1 Wikipedia page
googleplus
18 Google+ users
reddit
7 Redditors
video
3 video uploaders

Citations

dimensions_citation
55 Dimensions

Readers on

mendeley
161 Mendeley
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Title
Transcriptome profile analysis reflects rat liver and kidney damage following chronic ultra-low dose Roundup exposure
Published in
Environmental Health, August 2015
DOI 10.1186/s12940-015-0056-1
Pubmed ID
Authors

Robin Mesnage, Matthew Arno, Manuela Costanzo, Manuela Malatesta, Gilles-Eric Séralini, Michael N. Antoniou

Abstract

Glyphosate-based herbicides (GBH) are the major pesticides used worldwide. Converging evidence suggests that GBH, such as Roundup, pose a particular health risk to liver and kidneys although low environmentally relevant doses have not been examined. To address this issue, a 2-year study in rats administering 0.1 ppb Roundup (50 ng/L glyphosate equivalent) via drinking water (giving a daily intake of 4 ng/kg bw/day of glyphosate) was conducted. A marked increased incidence of anatomorphological and blood/urine biochemical changes was indicative of liver and kidney structure and functional pathology. In order to confirm these findings we have conducted a transcriptome microarray analysis of the liver and kidneys from these same animals. The expression of 4224 and 4447 transcript clusters (a group of probes corresponding to a known or putative gene) were found to be altered respectively in liver and kidney (p < 0.01, q < 0.08). Changes in gene expression varied from -3.5 to 3.7 fold in liver and from -4.3 to 5.3 in kidneys. Among the 1319 transcript clusters whose expression was altered in both tissues, ontological enrichment in 3 functional categories among 868 genes were found. First, genes involved in mRNA splicing and small nucleolar RNA were mostly upregulated, suggesting disruption of normal spliceosome activity. Electron microscopic analysis of hepatocytes confirmed nucleolar structural disruption. Second, genes controlling chromatin structure (especially histone-lysine N-methyltransferases) were mostly upregulated. Third, genes related to respiratory chain complex I and the tricarboxylic acid cycle were mostly downregulated. Pathway analysis suggests a modulation of the mTOR and phosphatidylinositol signalling pathways. Gene disturbances associated with the chronic administration of ultra-low dose Roundup reflect a liver and kidney lipotoxic condition and increased cellular growth that may be linked with regeneration in response to toxic effects causing damage to tissues. Observed alterations in gene expression were consistent with fibrosis, necrosis, phospholipidosis, mitochondrial membrane dysfunction and ischemia, which correlate with and thus confirm observations of pathology made at an anatomical, histological and biochemical level. Our results suggest that chronic exposure to a GBH in an established laboratory animal toxicity model system at an ultra-low, environmental dose can result in liver and kidney damage with potential significant health implications for animal and human populations.

Twitter Demographics

The data shown below were collected from the profiles of 309 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 161 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
Peru 1 <1%
Uruguay 1 <1%
Sri Lanka 1 <1%
Italy 1 <1%
Unknown 155 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 33 20%
Researcher 25 16%
Student > Master 23 14%
Student > Ph. D. Student 16 10%
Other 12 7%
Other 28 17%
Unknown 24 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 49 30%
Medicine and Dentistry 22 14%
Biochemistry, Genetics and Molecular Biology 14 9%
Environmental Science 12 7%
Pharmacology, Toxicology and Pharmaceutical Science 8 5%
Other 24 15%
Unknown 32 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 423. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2019.
All research outputs
#24,372
of 14,030,042 outputs
Outputs from Environmental Health
#11
of 1,119 outputs
Outputs of similar age
#504
of 239,118 outputs
Outputs of similar age from Environmental Health
#1
of 1 outputs
Altmetric has tracked 14,030,042 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,119 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.1. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 239,118 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them