Chapter title |
Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells
|
---|---|
Chapter number | 26 |
Book title |
Nanoscale Imaging
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8591-3_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8590-6, 978-1-4939-8591-3
|
Authors |
Lucia Gardini, Martino Calamai, Hiroyasu Hatakeyama, Makoto Kanzaki, Marco Capitanio, Francesco Saverio Pavone, Gardini, Lucia, Calamai, Martino, Hatakeyama, Hiroyasu, Kanzaki, Makoto, Capitanio, Marco, Pavone, Francesco Saverio |
Abstract |
Here, we describe protocols for three-dimensional tracking of single quantum dot-conjugated molecules with nanometer accuracy in living cells using conventional fluorescence microscopy. The technique exploits out-of-focus images of single emitters combined with an automated pattern-recognition open-source software that fits the images with proper model functions to extract the emitter coordinates. We describe protocols for targeting quantum dots to both membrane components and cytosolic proteins. |
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