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Validity of a commercial kit for detection of antibodies in bovine serum in an endemic area for fasciolosis

Overview of attention for article published in Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, June 2017
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Title
Validity of a commercial kit for detection of antibodies in bovine serum in an endemic area for fasciolosis
Published in
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, June 2017
DOI 10.1590/s1984-29612017016
Pubmed ID
Authors

Aline Nunes Simões, Sayanne Luns Hatum de Almeida, Áquila Flávia da Rocha Braga, Isabella Vilhena Freire Martins, Dirlei Molinari Donatele, Graziela Barioni

Abstract

Fasciolosis is caused by Fasciola hepatica that affects the bile ducts and liver parenchyma of ruminants, which can result in economic loss. This study aimed to carry out the validity of the commercial kit ELISA® indirect front of the simple fecal sedimentation test used as the standard. 143 samples were collected blood and feces of cattle from Jerome, south of the Espírito Santo. Serum samples were left at -80 °C and used to perform the ELISA kit IDEXX®. All animals to stool examinations were also positive to the ELISA (22) and negative samples to test stool (121), 52 animals reacted positively against the antibody research. The frequency of fasciolosis was 15.4% in the stool examinations and 51.8% by ELISA. The validity was calculated by sensitivity (100%), specificity (57%), positive predictive value (29%) and negative predictive value (100%), and the correlation between the tests calculated using the kappa index of 0.35. The better sensitivity of the ELISA commercial kit should not be a separately evaluated, since the cost benefit and the technical facility must be considered.

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Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 23%
Student > Bachelor 3 23%
Professor 1 8%
Lecturer > Senior Lecturer 1 8%
Researcher 1 8%
Other 1 8%
Unknown 3 23%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 54%
Agricultural and Biological Sciences 1 8%
Unknown 5 38%