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Switchable genome editing via genetic code expansion

Overview of attention for article published in Scientific Reports, July 2018
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (98th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

news
15 news outlets
blogs
4 blogs
twitter
68 X users
facebook
1 Facebook page

Citations

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11 Dimensions

Readers on

mendeley
88 Mendeley
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Title
Switchable genome editing via genetic code expansion
Published in
Scientific Reports, July 2018
DOI 10.1038/s41598-018-28178-3
Pubmed ID
Authors

Toru Suzuki, Maki Asami, Sanjay G. Patel, Louis Y. P. Luk, Yu-Hsuan Tsai, Anthony C. F. Perry

Abstract

Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light. However, these approaches are often impracticable, for example in clinical therapeutic genome editing in situ or gene drives in which environmentally-compatible control is paramount. With this in mind, we have developed heritable Cas9-mediated mammalian genome editing that is acutely controlled by the cheap lysine derivative, Lys(Boc) (BOC). Genetic code expansion permitted non-physiological BOC incorporation such that Cas9 (Cas9BOC) was expressed in a full-length, active form in cultured somatic cells only after BOC exposure. Stringently BOC-dependent, heritable editing of transgenic and native genomic loci occurred when Cas9BOC was expressed at the onset of mouse embryonic development from cRNA or Cas9BOC transgenic females. The tightly controlled Cas9 editing system reported here promises to have broad applications and is a first step towards purposed, spatiotemporal gene drive regulation over large geographical ranges.

X Demographics

X Demographics

The data shown below were collected from the profiles of 68 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 88 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 19%
Student > Ph. D. Student 16 18%
Student > Bachelor 13 15%
Other 11 13%
Student > Master 6 7%
Other 10 11%
Unknown 15 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 34%
Biochemistry, Genetics and Molecular Biology 25 28%
Chemistry 7 8%
Engineering 2 2%
Medicine and Dentistry 2 2%
Other 6 7%
Unknown 16 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 161. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2023.
All research outputs
#248,512
of 25,085,000 outputs
Outputs from Scientific Reports
#2,862
of 137,706 outputs
Outputs of similar age
#5,437
of 334,001 outputs
Outputs of similar age from Scientific Reports
#65
of 3,528 outputs
Altmetric has tracked 25,085,000 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 137,706 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.7. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 334,001 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 3,528 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.