| Chapter title |
AFM-Based Single-Molecule Force Spectroscopy of Proteins
|
|---|---|
| Chapter number | 3 |
| Book title |
Nanoscale Imaging
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8591-3_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8590-6, 978-1-4939-8591-3
|
| Authors |
Zackary N. Scholl, Piotr E. Marszalek |
| Abstract |
Single-molecule force spectroscopy by AFM (AFM-SMFS) is an experimental methodology that allows unequivocal sensitivity and control for investigating and manipulating the mechanical properties of single molecules. The past 20 years of AFM-SMFS has provided numerous breakthroughs in the understanding of the mechanical properties and force-induced structural rearrangements of sugars, DNA, and proteins. Here, we focus on the application of AFM-SMFS to study proteins, since AFM-SMFS has succeeded in providing abundant information about protein folding pathways, kinetics, interactions, and misfolding. In this chapter we describe the experimental procedures for conducting a SMFS-AFM experiment-including purification of protein samples, setup and calibration of the AFM instrumentation, and the thorough and unbiased analysis of resulting AFM data. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 25% |
| Student > Ph. D. Student | 2 | 25% |
| Unspecified | 1 | 13% |
| Other | 1 | 13% |
| Student > Master | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 25% |
| Business, Management and Accounting | 1 | 13% |
| Unspecified | 1 | 13% |
| Agricultural and Biological Sciences | 1 | 13% |
| Engineering | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 2 | 25% |