Chapter title |
Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged Using High-Speed AFM
|
---|---|
Chapter number | 13 |
Book title |
Nanoscale Imaging
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8591-3_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8590-6, 978-1-4939-8591-3
|
Authors |
Masayuki Endo, Hiroshi Sugiyama, Endo, Masayuki, Sugiyama, Hiroshi |
Abstract |
The visualization of biomolecules is a straightforward way to elucidate the physical properties of molecules and their reaction processes. Atomic force microscopy (AFM) enables the direct imaging of biomolecules under physiological conditions at nanometer-scale spatial resolution. Because AFM visualizes all molecules in a scanning area, an observation scaffold is required for the target-specific imaging of molecules in the dynamic state. The DNA origami technology allows the precise placement of target molecules in a designed nanostructure, and the detection of the molecules at the single-molecule level. DNA origami is applied for visualizing the detailed motions of molecules using high-speed AFM (HS-AFM), which enables the analysis of the dynamic movement of biomolecules in a subsecond time resolution. Here, we describe the combination of the DNA origami system with HS-AFM for the imaging of DNA structural changes controlled by photoresponsive molecules. The hybridization and dehybridization of photoresponsive oligonucleotides were visualized directly using this observation system. These target-oriented observation systems should contribute to the detailed analysis of biomolecules in real time with molecular resolution. |
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