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Nanoscale Imaging

Overview of attention for book
Cover of 'Nanoscale Imaging'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 High-Resolution Atomic Force Microscopy Imaging of Nucleic Acids
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    Chapter 2 Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing
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    Chapter 3 AFM-Based Single-Molecule Force Spectroscopy of Proteins
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    Chapter 4 High-Resolution AFM-Based Force Spectroscopy
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    Chapter 5 Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions
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    Chapter 6 Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers
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    Chapter 7 AFM-Based Characterization of Electrical Properties of Materials
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    Chapter 8 Supported Lipid Bilayers for Atomic Force Microscopy Studies
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    Chapter 9 Quantifying Small Molecule Binding Interactions with DNA Nanostructures
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    Chapter 10 Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy
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    Chapter 11 High-Resolution and High-Speed Atomic Force Microscope Imaging
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    Chapter 12 High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies
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    Chapter 13 Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged Using High-Speed AFM
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    Chapter 14 Assembly of Centromere Chromatin for Characterization by High-Speed Time-Lapse Atomic Force Microscopy
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    Chapter 15 High-Speed Force Spectroscopy for Single Protein Unfolding
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    Chapter 16 Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assays
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    Chapter 17 Preparing Frozen-Hydrated Protein–Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging
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    Chapter 18 Probing Chromatin Structure with Magnetic Tweezers
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    Chapter 19 Single-Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly
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    Chapter 20 Correlative Atomic Force and Single-Molecule Fluorescence Microscopy of Nucleoprotein Complexes
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    Chapter 21 Sensing the Ultrastructure of Bacterial Surfaces and Their Molecular Binding Forces Using AFM
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    Chapter 22 Nanoscale Visualization of Bacterial Microcompartments Using Atomic Force Microscopy
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    Chapter 23 Time-Resolved Imaging of Bacterial Surfaces Using Atomic Force Microscopy
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    Chapter 24 Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy
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    Chapter 25 Fluorescence Correlation Spectroscopy on Genomic DNA in Living Cells
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    Chapter 26 Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells
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    Chapter 27 AFM Indentation Analysis of Cells to Study Cell Mechanics and Pericellular Coat
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    Chapter 28 Imaging of Soft and Biological Samples Using AFM Ringing Mode
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    Chapter 29 Probing Single Virus Binding Sites on Living Mammalian Cells Using AFM
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    Chapter 30 Applications of Atomic Force Microscopy for Adhesion Force Measurements in Mechanotransduction
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    Chapter 31 Methods for Atomic Force Microscopy of Biological and Living Specimens
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    Chapter 32 Single Molecule Imaging in Live Embryos Using Lattice Light-Sheet Microscopy
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    Chapter 33 Silver Filler Pre-embedding to Enhance Resolution and Contrast in Multidimensional SEM: A Nanoscale Imaging Study on Liver Tissue
  35. Altmetric Badge
    Chapter 34 Nanoscale Dynamics and Energetics of Proteins and Protein-Nucleic Acid Complexes in Classical Molecular Dynamics Simulations
Attention for Chapter 16: Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assays
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Chapter title
Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assays
Chapter number 16
Book title
Nanoscale Imaging
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8591-3_16
Pubmed ID
Book ISBNs
978-1-4939-8590-6, 978-1-4939-8591-3
Authors

Mohamed Fareh, Chirlmin Joo, Fareh, Mohamed, Joo, Chirlmin

Abstract

Recent advances in single-molecule techniques allow for dynamic observations of the interactions between various protein assemblies and RNA molecules with high spatiotemporal resolution. However, it remains challenging to obtain functional eukaryotic protein complexes and cost-effective fluorescently labeled RNAs to study their interactions at the single-molecule level. Here, we describe protocols combining single-molecule fluorescence with various protein complex pull-down techniques to determine the function of RNA-interacting protein complexes of interest. We provide step-by-step guidance for using novel single-molecule techniques including RNA labeling, protein complexes purification, and single-molecule imaging. As a proof-of-concept of the utility of our single-molecule approaches, we show how human Dicer and its cofactor TRBP orchestrate the biogenesis of microRNA in real time. These single-molecule pull-down and fluorescence assays provide sub-second time resolution and can be applied to various ribonucleoprotein complexes that are essential for cellular processes.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 33%
Student > Ph. D. Student 1 17%
Student > Bachelor 1 17%
Student > Master 1 17%
Other 1 17%
Other 0 0%
Readers by discipline Count As %
Engineering 2 33%
Agricultural and Biological Sciences 1 17%
Biochemistry, Genetics and Molecular Biology 1 17%
Unknown 2 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 June 2018.
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#12,217,631
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Outputs from Methods in molecular biology
#6,044
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#232,959
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Outputs of similar age from Methods in molecular biology
#1
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