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Nanoscale Imaging

Overview of attention for book
Cover of 'Nanoscale Imaging'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 High-Resolution Atomic Force Microscopy Imaging of Nucleic Acids
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    Chapter 2 Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing
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    Chapter 3 AFM-Based Single-Molecule Force Spectroscopy of Proteins
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    Chapter 4 High-Resolution AFM-Based Force Spectroscopy
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    Chapter 5 Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions
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    Chapter 6 Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers
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    Chapter 7 AFM-Based Characterization of Electrical Properties of Materials
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    Chapter 8 Supported Lipid Bilayers for Atomic Force Microscopy Studies
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    Chapter 9 Quantifying Small Molecule Binding Interactions with DNA Nanostructures
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    Chapter 10 Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy
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    Chapter 11 High-Resolution and High-Speed Atomic Force Microscope Imaging
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    Chapter 12 High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies
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    Chapter 13 Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged Using High-Speed AFM
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    Chapter 14 Assembly of Centromere Chromatin for Characterization by High-Speed Time-Lapse Atomic Force Microscopy
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    Chapter 15 High-Speed Force Spectroscopy for Single Protein Unfolding
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    Chapter 16 Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assays
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    Chapter 17 Preparing Frozen-Hydrated Protein–Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging
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    Chapter 18 Probing Chromatin Structure with Magnetic Tweezers
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    Chapter 19 Single-Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly
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    Chapter 20 Correlative Atomic Force and Single-Molecule Fluorescence Microscopy of Nucleoprotein Complexes
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    Chapter 21 Sensing the Ultrastructure of Bacterial Surfaces and Their Molecular Binding Forces Using AFM
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    Chapter 22 Nanoscale Visualization of Bacterial Microcompartments Using Atomic Force Microscopy
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    Chapter 23 Time-Resolved Imaging of Bacterial Surfaces Using Atomic Force Microscopy
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    Chapter 24 Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy
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    Chapter 25 Fluorescence Correlation Spectroscopy on Genomic DNA in Living Cells
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    Chapter 26 Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells
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    Chapter 27 AFM Indentation Analysis of Cells to Study Cell Mechanics and Pericellular Coat
  29. Altmetric Badge
    Chapter 28 Imaging of Soft and Biological Samples Using AFM Ringing Mode
  30. Altmetric Badge
    Chapter 29 Probing Single Virus Binding Sites on Living Mammalian Cells Using AFM
  31. Altmetric Badge
    Chapter 30 Applications of Atomic Force Microscopy for Adhesion Force Measurements in Mechanotransduction
  32. Altmetric Badge
    Chapter 31 Methods for Atomic Force Microscopy of Biological and Living Specimens
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    Chapter 32 Single Molecule Imaging in Live Embryos Using Lattice Light-Sheet Microscopy
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    Chapter 33 Silver Filler Pre-embedding to Enhance Resolution and Contrast in Multidimensional SEM: A Nanoscale Imaging Study on Liver Tissue
  35. Altmetric Badge
    Chapter 34 Nanoscale Dynamics and Energetics of Proteins and Protein-Nucleic Acid Complexes in Classical Molecular Dynamics Simulations
Attention for Chapter 19: Single-Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)

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Chapter title
Single-Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly
Chapter number 19
Book title
Nanoscale Imaging
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8591-3_19
Pubmed ID
Book ISBNs
978-1-4939-8590-6, 978-1-4939-8591-3
Authors

Jaya Sarkar, Sua Myong, Sarkar, Jaya, Myong, Sua

Abstract

Ribonucleoprotein (RNP) granules are membraneless organelles, consisting of high local concentrations of RNA and proteins bearing intrinsically disordered regions (IDRs). They are formed by liquid-liquid phase separation (LLPS). In neurodegenerative diseases such as ALS, mutations in granule proteins such as FUS and TDP-43 accelerate abnormal liquid to solid transition of RNP granules, leading to formation of fiber-like structures. Methods to study granules must be carefully selected based on the stage of granule's life. Here we describe a strategic combination of single-molecule biophysical and ensemble biochemical techniques that may be employed to extract insightful information about early stages of RNP granule formation. Protein-RNA interaction and stoichiometry of the complex in the early soluble stage of RNP assembly can be probed by single-molecule FRET (smFRET) assay and electrophoretic mobility shift assay (EMSA), respectively. RNP-RNP interaction that likely contributes to RNP nucleation can be reported on by a smFRET-based RNA annealing assay. The next stage in the assembly pathway, that is, phase separation from diffused to liquid-like droplets, may be monitored by a phase separation assay. Finally, RNP granules isolated from mammalian cells can be investigated using a unique single-molecule pull-down (SiMPull) assay.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 36%
Student > Doctoral Student 2 18%
Professor 1 9%
Student > Master 1 9%
Researcher 1 9%
Other 0 0%
Unknown 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 45%
Agricultural and Biological Sciences 2 18%
Neuroscience 2 18%
Unknown 2 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2018.
All research outputs
#2,112,212
of 15,948,653 outputs
Outputs from Methods in molecular biology
#441
of 9,297 outputs
Outputs of similar age
#56,467
of 278,147 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
Altmetric has tracked 15,948,653 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,297 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,147 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them