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Mendeley readers
Chapter title |
Fluorescence Correlation Spectroscopy on Genomic DNA in Living Cells
|
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Chapter number | 25 |
Book title |
Nanoscale Imaging
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-8591-3_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8590-6, 978-1-4939-8591-3
|
Authors |
Cameron Hodges, Jens-Christian Meiners, Hodges, Cameron, Meiners, Jens-Christian |
Abstract |
Fluorescence correlation spectroscopy (FCS) is a powerful technique used to measure diffusion, fluctuations, and other transport processes in biomolecular systems. It is, however, prone to artifacts and subject to considerable experimental difficulties when applied to living cells. In this chapter, we provide protocols to conduct quantitative FCS measurements on DNA inside living eukaryotic and prokaryotic cells. We discuss sample preparation, dye selection and characterization, FCS data acquisition, and data analysis, including a method to com pensate for photobleaching to obtain quantitatively meaningful spectra. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 43% |
Professor > Associate Professor | 1 | 14% |
Student > Bachelor | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Agricultural and Biological Sciences | 2 | 29% |
Chemistry | 1 | 14% |
Unknown | 2 | 29% |